CLUSTERED PRIMARY BRANCH 1, a new allele of DWARF11, controls panicle architecture and seed size in rice
CLUSTERED PRIMARY BRANCH 1, a new allele of DWARF11, controls panicle architecture and seed size in rice
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CLUSTERED PRIMARY BRANCH 1 是 DWARF11 的新等位基因，控制水稻的穗结构和种子大小
DOI：
10.1111/pbi.12391
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发表时间：
2016
影响因子：
13.8
通讯作者：
Tan Lubin
中科院分区：
文献类型：
--
作者：
Wu Yongzhen;Fu Yongcai;Zhao Shuangshuang;Gu Ping;Zhu Zuofeng;Sun Chuanqing;Tan Lubin
穗型结构和种子大小是直接决定水稻产量的重要农艺性状。虽然近年来已经克隆和鉴定了许多控制穗型结构和种子大小的关键基因，但其遗传和分子机制尚不清楚。在这项研究中，我们发现了一个突变体，产生的穗具有束状的初级分支和缩小的种子大小。我们分离出了下层的clustered PRIMARY BRANCH 1（CPB1）基因，这是dwarf11 （D11）的一个新的等位基因，编码参与油菜素内酯（BR）生物合成途径的细胞色素P450蛋白。遗传转化实验证实，位于CPB1/D11高度保守区域的His360Leu氨基酸取代导致了pb1突变体的穗结构和种子大小的变化。在cpb1突变背景下，cpb1 / d11过表达不仅挽救了正常的穗型结构和株高，而且叶片角和种子大小也比对照大。此外，由穗特异性启动子驱动的pb1 / d11转基因植株可以在不影响其他有利农艺性状的情况下扩大种子大小和提高籽粒产量。这些结果表明，cpb1 / d11突变影响了水稻穗型结构和籽粒大小的发育，利用cpb1 / d11启动子调控cpb1 / d11的表达可以提高水稻籽粒大小，从而提高产量。
Panicle architecture and seed size are important agronomic traits that directly determine grain yield in rice (Oryza sativaL.). Although a number of key genes controlling panicle architecture and seed size have been cloned and characterized in recent years, their genetic and molecular mechanisms remain unclear. In this study, we identified a mutant that produced panicles with fascicled primary branching and reduced seeds in size. We isolated the underlyingCLUSTERED PRIMARY BRANCH 1(CPB1) gene, a new allele ofDWARF11(D11) encoding a cytochrome P450 protein involved in brassinosteroid (BR) biosynthesis pathway. Genetic transformation experiments confirmed that a His360Leu amino acid substitution residing in the highly conserved region of CPB1/D11 was responsible for the panicle architecture and seed size changes in thecpb1mutants. Overexpression ofCPB1/D11under the background ofcpb1mutant not only rescued normal panicle architecture and plant height, but also had a larger leaf angle and seed size than the controls. Furthermore, theCPB1/D11transgenic plants driven by panicle‐specific promoters can enlarge seed size and enhance grain yield without affecting other favourable agronomic traits. These results demonstrated that the specific mutation inCPB1/D11influenced development of panicle architecture and seed size, and manipulation ofCPB1/D11expression using the panicle‐specific promoter could be used to increase seed size, leading to grain yield improvement in rice.