Ionomic Profiling of Rice Genotypes and Identification of Varieties with Elemental Covariation Effects
Ionomic Profiling of Rice Genotypes and Identification of Varieties with Elemental Covariation Effects
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DOI：
10.1016/j.rsci.2021.12.007
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发表时间：
2022-01
期刊：
影响因子：
4.8
通讯作者：
Chengming Zhang;N. Tanaka;M. S. Dwiyanti;M. Shenton;Hayato Maruyama;T. Shinano;Q. Chu;Xie Jun;Toshihiro Watanabe
中科院分区：
文献类型：
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作者：
Chengming Zhang;N. Tanaka;M. S. Dwiyanti;M. Shenton;Hayato Maruyama;T. Shinano;Q. Chu;Xie Jun;Toshihiro Watanabe
离子谱主要受遗传和环境因素的影响。识别对品种效应的离子反应对于了解物种或亚种之间的离子变异以及潜在地了解遗传对离子谱的影响是必要的。在相同的水培条件下，将120个水稻品种培育到苗期，测定了水稻地上部和根部26种元素(包括3种阴离子)的含量。虽然亚种效应受到稻属骨架及其元素化学性质的限制，但我们发现亚种间多数元素的离子变异在亚种间存在显著差异。相关性的主成分分析表明，根-冠离子运输机制的差异是造成亚种间离子差异的主要原因。此外，这些相关性主要与筛选元素协变效应的品种有关，这有助于培育具有安全浓度的其他有毒元素的生物强化水稻品种。日本亚种表现出最强的元素相关性和元素协变效应，因此，它们在生物强化方面表现出比水稻和其他亚种更大的优势，而在金属(Loid)污染土壤中，水稻和其他亚种可能更安全。我们还发现，地理和历史分布显著地决定了离子剖面。总体而言，本研究结果为进一步开展相关研究以改善稻米生产中的营养状况和降低毒性风险提供了参考。
Ionomic profiles are primarily influenced by genetic and environmental factors. Identifying ionomic responses to varietal effects is necessary to understand the ionomic variations among species or subspecies and to potentially understand genetic effects on ionomic profiles. We cultivated 120 rice (Oryza sativa) varieties to seedling stage in identical hydroponic conditions and determined the concentrations of 26 elements (including 3 anions) in the shoots and roots of rice. Although the subspecies effects were limited by the genusOryzapre-framework and its elemental chemical properties, we found significant differences in ionomic variations in most elements among theaus,indicaandjaponicasubspecies. Principal component analysis of the correlations indicated that variations in the root-to-shoot ionomic transport mechanisms were the main causes of ionomic differences among the subspecies. Furthermore, the correlations were primarily associated with the screening of varieties for elemental covariation effects that can facilitate breeding biofortified rice varieties with safe concentrations of otherwise toxic elements. Thejaponicasubspecies exhibited the strongest elemental correlations and elemental covariation effects, therefore, they showed greater advantages for biofortification than theindicaandaussubspecies, whereasindicaandaussubspecies were likely safer in metal(loid) polluted soils. We also found that geographical and historical distribution significantly defined the ionomic profiles. Overall, the results of this study provided a reference for further association studies to improve the nutritional status and minimize toxicity risks in rice production.