Adenovirus-mediated gene transfer of a heat shock protein 70 (hsp 70i) protects against simulated ischemia.
Adenovirus-mediated gene transfer of a heat shock protein 70 (hsp 70i) protects against simulated ischemia.
复制标题
腺病毒介导的热休克蛋白 70 (hsp 70i) 基因转移可防止模拟缺血。
DOI：
10.1006/jmcc.1996.0228
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Dillmann,WH
中科院分区：
文献类型：
--
作者：
Mestril,R;Giordano,FJ;Conde,AG;Dillmann,WH
We have recently shown that the overexpression of a heat shock protein 70 (hsp70) in a rat myogenic cell line confers protection against simulated ischemia. We also developed and demonstrated that overexpression of this protein, in the hearts of transgenic mice, protects against ischemia/reperfusion injury. We have now inserted the hsp70 gene in an adenoviral vector and show that we are able to transfer and achieve overexpression of this protein in neonatal cardiomyocytes and in the rat myogenic cell line H9c2. We find that cells infected with the adenoviral-hsp70i construct are rendered tolerant to simulated ischemia as compared to cells infected with a control recombinant adenoviral construct. In conclusion, our results demonstrate the feasibility of using adenoviral vectors to overexpress the hsp70 in myogenic cells, specially in cardiomyocytes, and the efficiency of this approach for providing protection against myocardial ischemia.
