H2S aids osmotic stress resistance by S-sulfhydration of melatonin production-related enzymes in Arabidopsis thaliana
H2S aids osmotic stress resistance by S-sulfhydration of melatonin production-related enzymes in Arabidopsis thaliana
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DOI：
10.1007/s00299-021-02813-2
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发表时间：
2021-11
影响因子：
6.2
通讯作者：
Zhiqing Wang;Y. Mu;Xuefeng Hao;Jinbao Yang;Daixuan Zhang;Zhuping Jin;Y. Pei
中科院分区：
文献类型：
--
作者：
Zhiqing Wang;Y. Mu;Xuefeng Hao;Jinbao Yang;Daixuan Zhang;Zhuping Jin;Y. Pei
Key messageHydrogen sulfide closedArabidopsis thalianastomata by increasing the transcription of melatonin-producing enzymes and the post-translational modification levels to combat osmotic stress.AbstractHydrogen sulfide (H2S) and melatonin (MEL) reportedly have similar functions in many aspects of plant growth, development and stress response. They regulate stomatal movement and enhance drought resistance. However, their physiological relationship is not well understood. Here, their crosstalk involved in osmotic stress resistance inArabidopsis thalianawas studied. Exogenous H2S and MEL closed stomata under normal or osmotic stress conditions and increased the relative water contents of plants under osmotic stress conditions. At the same time, exogenous H2S and MEL responded to osmotic stress by increasing the content of proline and soluble sugar, and reducing malondialdehyde (MDA) content and relative conductivity. Using mutants in the MEL-associated production of serotoninN-acetyltransferase (snat), caffeic acidO-methyltransferase (comt1) andN-acetylserotonin methyltransferase (asmt), we determined that H2S was partially dependent on MEL to close stomata. Additionally, the overexpression ofASMTpromoted stomatal closure. Exogenous H2S increased the transcription levels of SNAT, ASMT and COMT1. Furthermore, exogenous H2S treatments increased the endogenous MEL content significantly. At the post-translational level, H2S sulfhydrated the SNAT and ASMT, but not COMT1, enzymes associated with MEL production. Thus, H2S appeared to promote stomatal closure in response to osmotic stress by increasing the transcription levels of MEL synthesis-related genes and the sulfhydryl modification of the encoded enzymes. These results increased our understanding of H2S and MEL functions and interactions under osmotic stress conditions.
