Transduction of neurons lining the cerebral external capsules in mice with feline immunodeficiency virus based vectors
Transduction of neurons lining the cerebral external capsules in mice with feline immunodeficiency virus based vectors
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DOI：
10.1016/s0304-3940(03)00833-4
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发表时间：
2003-11-13
影响因子：
2.5
通讯作者：
Davidson, BL
中科院分区：
文献类型：
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作者：
Alisky, JM;Hughes, SM;Davidson, BL
基因治疗在大脑中主要集中在神经元（灰质）上，对白色物质的研究很少。在这项研究中，将小鼠的大脑白色物质注射到小鼠中，以评估表达β-半乳糖苷酶或β-葡萄糖醛酸苷酶的重组猫免疫缺陷病毒载体的基因转移的分布。我们的研究结果表明，载体优先分布沿着的白色物质的外囊，这是网站的载体注射证实辣根过氧化物酶标记。此外，我们发现基因几乎只转移到外囊内衬的NeuN（+）细胞，然后这些细胞在注射侧整个外囊的白色物质中强烈分泌重组β-葡萄糖醛酸酶。这些结果可能适用于广泛的中枢神经系统缺陷的溶酶体贮积病，以及其他疾病，如多发性硬化症和人类免疫缺陷病毒痴呆症。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
Gene therapy in the brain has focused mainly on neurons (gray matter), with little comparable research on white matter. in this study, injections into mice cerebral white matter of mice were done to assess the distribution of gene transfer with recombinant feline immunodeficiency virus vectors expressing either beta-galactosidase or beta-glucuronidase. Our results show that vectors were preferentially distributed along the white matter of the external capsule, which was the site of vector injection as confirmed by horseradish peroxidase labeling. Moreover, we found gene transfer almost exclusively to NeuN(+) cells lining the external capsule, which then robustly secreted recombinant beta-glucuronidase throughout the white matter of the entire external capsule on the injected side. These results may have application to lysosomal storage diseases with widespread central nervous system deficits, and other disorders such as multiple sclerosis and human immunodeficiency virus dementia. (C) 2003 Elsevier Ireland Ltd. All rights reserved.