In vivo bioluminescence imaging study to monitor ectopic bone formation by luciferase gene marked mesenchymal stem cells
In vivo bioluminescence imaging study to monitor ectopic bone formation by luciferase gene marked mesenchymal stem cells
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DOI：
10.1002/jor.20582
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发表时间：
2008-07-01
影响因子：
2.8
通讯作者：
Martens, Anton C. M.
中科院分区：
文献类型：
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作者：
Olivo, Cristina;Alblas, Jacqueline;Martens, Anton C. M.
间充质干细胞（MSC）是再生医学应用的有力工具。在这项研究中，我们使用体内生物发光成像非侵入性地调查的命运和组织工程结构中的成人骨髓间充质干细胞骨形成的贡献。将表达GFP-荧光素酶的山羊MSC接种在陶瓷支架上并皮下植入免疫缺陷小鼠中。每周用生物发光成像监测构建体，7周后取出，通过组织形态计量学定量骨形成。随着接种MSC量的增加（从0到1 x 10（6）MSC/支架），在所有时间点观察到生物发光的细胞剂量相关增加，与7周时骨形成增加相关。为了研究MSC增殖与骨沉积的相关性，对细胞接种的支架进行辐照。辐照的细胞在耗氧量方面是有功能的，但在体内没有观察到生物发光的增加，并且仅产生最小的骨。骨髓间充质干细胞可能是体内组织工程构建体中骨形成起始所必需的。生物发光是监测细胞反应和预测体内骨形成的有用工具。(C)2008骨科研究学会。由威利期刊公司出版
Mesenchymal stem cells (MSCs) represent a powerful tool for applications in regenerative medicine. In this study, we used in vivo bioluminescence imaging to noninvasively investigate the fate and the contribution to bone formation of adult MSCs in tissue engineered constructs. Goat MSCs expressing GFP-luciferase were seeded on ceramic scaffolds and implanted subcutaneously in immune-deficient mice. The constructs were monitored weekly with bioluminescence imaging and were retrieved after 7 weeks to quantify bone formation by histomorphometry. With increasing amounts of seeded MSCs (from 0 to 1 x 10(6) MSC/scaffold), a cell-dose related increase in bioluminescence was observed at all time points, correlating with increased bone formation at 7 weeks. To investigate the relevance of MSC proliferation to bone deposition, cell-seeded scaffolds were irradiated. The irradiated cells were functional with respect to oxygen consumption but no increase in bioluminescence was observed in vivo, and only minimal bone was produced. Proliferating MSCs are likely required for initiation of bone formation in tissue engineered constructs in vivo. Bioluminescence is a useful tool to monitor cellular responses and predict bone formation in vivo. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.