Recovery of replication-competent HIV despite prolonged suppression of plasma viremia
Recovery of replication-competent HIV despite prolonged suppression of plasma viremia
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DOI：
10.1126/science.278.5341.1291
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发表时间：
1997-11-14
期刊：
影响因子：
56.9
通讯作者：
Richman, DD
中科院分区：
文献类型：
--
作者：
Wong, JK;Hezareh, M;Richman, DD
在评估目前治疗人类免疫缺陷病毒(HIV)疾病的联合用药方案时，确定病毒库的存在是重要的。在去除患者和正常献血者外周血单个核细胞(PBMC)中的CD8细胞后，用CD3和CD28的固定化抗体激活患者的CD4淋巴细胞，能够从6名患者的PBMC中分离出病毒，尽管他们的血浆HIV RNA在长达2年的时间里被抑制到每毫升低于50拷贝。HIV Poll的部分测序显示没有新的耐药性突变或明显的进化，提供了病毒潜伏而不是药物失败的证据。
In evaluating current combination drug regimens for treatment of human immunodeficiency virus (HIV) disease, it is important to determine the existence of viral reservoirs. After depletion of CD8 cells from the peripheral blood mononuclear cells (PBMCs) of both patients and normal donors, activation of patient CD4 lymphocytes with immobilized antibodies to CD3 and CD28 enabled the isolation of virus from PBMCs of six patients despite the suppression of their plasma HIV RNA to fewer than 50 copies per milliliter for up to 2 years. Partial sequencing of HIV pol revealed no new drug resistance mutations or discernible evolution, providing evidence for viral latency rather than drug failure.