Antibiotic resistant bacteria as bio-indicator of polluted effluent in the green turtles, Chelonia mydas in Oman
Antibiotic resistant bacteria as bio-indicator of polluted effluent in the green turtles, Chelonia mydas in Oman
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DOI：
10.1016/j.marenvres.2010.12.005
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发表时间：
2011-03-01
影响因子：
3.3
通讯作者：
Al-Alawi, Wafaa
中科院分区：
文献类型：
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作者：
Al-Bahry, Saif N.;Mahmoud, Ibrahim Y.;Al-Alawi, Wafaa
研究了抗生素抗性细菌作为绿海龟产卵期间海洋污染污水的生物指示剂。阿曼哈德角是世界上最重要的筑巢海滩之一，采用非侵入性输卵管液取样程序来测试海龟是否暴露于污染环境。每个样本都是通过将 15 厘米无菌拭子轻轻插入泄殖腔排气口，同时括约肌放松且泄殖腔内壁展开至外部来获得的。对四十只海龟进行了采样。分离出7属132种细菌。主要分离株是柠檬酸杆菌。分离株中 60.6% 对 15 种测试抗生素具有多重耐药性。对抗生素的主要耐药性是氨苄西林，其次是链霉素和磺胺甲恶唑。对输卵管液进行取样以检测对抗生素具有抗药性的细菌是评估海龟在进食和迁徙过程中接触污染污水的宝贵方法。使用细菌作为生物指示剂的污染污水可能会影响这种濒危物种的繁殖潜力。 (C) 2010 Elsevier Ltd. 保留所有权利。
Antibiotic resistant bacteria were studied as bio-indicators of marine polluted effluents during egg-laying in green turtles. A non-invasive procedure for sampling oviductal fluid was used to test for exposure of turtles to pollution in Ras Al-Hadd, Oman, which is one of the most important nesting beaches in the world. Each sample was obtained by inserting a 15 cm sterile swab gently into the cloacal vent as the sphincter muscle is relaxed and the cloacal lining is unfolded to the outside. Forty turtles were sampled. A hundred and thirty-two species of bacteria from 7 genera were isolated. The dominant isolate was Citrobacter. Among the isolates 60.6% were multiple resistant to 15 tested antibiotics. The dominant resistance to antibiotics was ampicillin followed by streptomycine and sulphamethoxazole. Sampling oviductal fluid for resistant bacteria to antibiotics is valuable way to assess exposure to polluted effluents during feeding and migratory in turtles. Polluted effluents using bacteria as bio-indicator may influence reproductive potential in this endangered species. (C) 2010 Elsevier Ltd. All rights reserved.