ISOLATION AND CHARACTERIZATION OF THE ZWF1 GENE OF SACCHAROMYCES-CEREVISIAE, ENCODING GLUCOSE-6-PHOSPHATE-DEHYDROGENASE
ISOLATION AND CHARACTERIZATION OF THE ZWF1 GENE OF SACCHAROMYCES-CEREVISIAE, ENCODING GLUCOSE-6-PHOSPHATE-DEHYDROGENASE
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DOI：
10.1016/0378-1119(90)90248-p
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发表时间：
1990-12-15
期刊：
影响因子：
3.5
通讯作者：
JOHNSTON, M
中科院分区：
文献类型：
--
作者：
NOGAE, I;JOHNSTON, M
葡萄糖-6-磷酸脱氢酶（G6PD）催化戊糖磷酸途径的第一步，该反应产生NADPH。我们分离到了编码G6PD的酿酒酵母基因ZWF1，并鉴定了其转录本和转录起点。ZWF1的表达似乎不受调控，这与它的“管家”作用一致。缺乏G6PD的零突变体似乎生长正常，但比野生型对氧化剂更敏感，这可能会降低NADPH的水平。这表明G6PD在酵母中NADPH的产生中起主要作用。在zwf1突变体中GAL1的表达调节正常，表明戊糖磷酸途径不参与葡萄糖抑制。预测酵母G6PD的氨基酸序列与果蝇、人类和大鼠的酶序列高度相似，但在其N端附近，酵母和果蝇的序列与人类和大鼠的序列不同。
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the firs step of the pentose phosphate pathway, a reaction that generates NADPH. We have isolated ZWF1, the Saccharomyces cerevisiae gene that encodes G6PD, and identified its transcript and transcription start point. Expression of ZWF1 appears not to be regulated, consistent with its ''housekeeping'' role. Null mutants lacking G6PD appear to grow normally, but are more sensitive than wild type to oxidizing agents that presumably reduce the level of NADPH. This suggests that G6PD has a major role in NADPH production in yeast. Regulation of GAL1 expression appears normal in zwf1 mutants, suggesting that the pentose phosphate pathway is not involved in glucose repression. The predicted amino acid sequence of yeast G6PD is highly similar to the sequence of the Drosophila, human, and rat enzymes, except near its N terminus, where the yeast and Drosophila sequences diverge from that of human and rat.