Fate of implanted syngenic muscle precursor cells in striated urethral sphincter of female rats: Perspectives for treatment of urinary incontinence
Fate of implanted syngenic muscle precursor cells in striated urethral sphincter of female rats: Perspectives for treatment of urinary incontinence
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DOI：
10.1016/j.urology.2004.06.058
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发表时间：
2004-11-01
期刊：
影响因子：
2.1
通讯作者：
Sebille, A
中科院分区：
文献类型：
--
作者：
Peyromaure, M;Sebe, P;Sebille, A
目标.分析同系骨骼肌前体细胞（MPCs）植入雌性大鼠横纹尿道括约肌后的结局。从下肢横纹肌中分离出MPC，并用携带绿色荧光蛋白基因的逆转录病毒感染。使用10-穆尔汉密尔顿注射器将大约10（5）个细胞纵向注射到24只动物的横纹尿道括约肌中。分别于术后0、1、7、10、14、30、90 d取全尿道行组织学观察和荧光分析。在第0天和第1天，在注射部位观察到一些小的圆形荧光MPC。在第7天，注意到显著的MPC持续存在，整个尿道壁（横纹肌层、平滑肌层和结缔组织）中有炎性细胞浸润。在第10天，横纹肌层中出现一些梭形细胞，表明MPCs掺入横纹肌纤维中。炎症细胞不再可见。第14天，梭形细胞趋向于更大。小而圆的细胞已经看不到了。在第30天和第90天，横纹肌层的所有肌纤维都有强荧光，而平滑肌层没有检测到荧光。在尿道括约肌中植入骨骼MPCs导致选择性掺入横纹肌纤维。肌源性细胞自体移植可能是治疗人类尿失禁的一种新方法。(C)2004年爱思唯尔公司
Objectives. To analyze the outcome of syngenic skeletal muscle precursor cells (MPCs) after implantation in the striated urethral sphincter of the female rat.Methods. MPCs were isolated from the striated muscles of the lower limbs and infected with a retrovirus carrying the gene for green fluorescent protein. Approximatively 10(5) cells were injected longitudinally in the striated urethral sphincter of 24 animals using a 10-muL Hamilton syringe. The whole urethra was excised at 0, 1, 7, 10, 14, 30, and 90 days after implantation for histologic study and fluorescence analysis of the transections.Results. At days 0 and 1, some small, round, fluorescent MPCs were observed at the injection site. At day 7, significant MPC persistence was noted, with infiltration of inflammatory cells in the whole urethral wall (striated muscle layer, smooth muscle layer, and connective tissue). At day 10, some fusiform cells appeared in the striated muscle layer, suggesting the incorporation of MPCs into the striated myofibers. Inflammatory cells were no longer visible. At day 14, the fusiform cells tended to be larger. The small, round cells were no longer seen. At days 30 and 90, all myofibers of the striated muscle layer were strongly fluorescent, and no fluorescence was detectable in the smooth muscle layer.Conclusions. Implantation of skeletal MPCs in the urethral sphincter resulted in selective incorporation into striated myofibers. Muscle-derived cell autografting could represent a new approach for the treatment of urinary incontinence in humans. (C) 2004 Elsevier Inc.