Magnetic circular dichroic spectra of cobalt(II) substituted metalloenzymes.
Magnetic circular dichroic spectra of cobalt(II) substituted metalloenzymes.
复制标题
钴（II）取代的金属酶的磁性圆二向色光谱。
DOI：
10.1021/bi00678a016
复制
发表时间：
1975
期刊：
影响因子：
2.9
通讯作者：
B. Vallée
中科院分区：
文献类型：
--
作者：
B. Holmquist;T. Kaden;B. Vallée
已经研究了钴 (II) 取代金属酶的磁性圆二色性 (MCD) 光谱，并与之前研究的一系列四、五和六配位钴 (II) 模型复合物进行了比较（T. A. Kaden 等人 (1974), Inorg. Chem. 13, 2582）。钴取代的羧肽酶 A、羧肽酶原 ta 和嗜热菌蛋白酶的 MCD 谱与早期从吸收谱中推论的四面体配位一致，也与 X 射线结构分析一致。抑制剂无法显着改变其 MCD 光谱。钴碱性磷酸酶和碳酸酐酶的 MCD 谱更为复杂，它们的 pH 依赖性和抑制剂的改变根据已知的钴 (II) 模型进行讨论。
The magnetic circular dichroic (MCD) spectra of cobalt(II) sugstituted metalloenzymes have been studied and compared to a series of four-, five-, and six-coordinate cobalt(II) model complexes previously examined (T. A. Kaden et al. (1974), Inorg. Chem. 13, 2582). The MCD spectra of cobalt substituted carboxypeptidase A, procarboxypeptidase ta, and thermolysin are consistent with earlier deductions of tetrahedral coordination from absorption spectra and also with X-ray structure analysis. Inhibitors fail to alter their MCD spectra significantly. The MCD spectra of cobalt alkaline phosphatase and carbonic anhydrase are more complex and their pH dependence and alteration by inhibitors are discussed in terms of known cobalt(II) models.