Transcriptome Analysis of Floral Buds Deciphered an Irregular Course of Meiosis in Polyploid Brassica rapa.
Transcriptome Analysis of Floral Buds Deciphered an Irregular Course of Meiosis in Polyploid Brassica rapa.
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花芽转录组分析破译了多倍体油菜减数分裂的不规则过程
DOI：
10.3389/fpls.2017.00768
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wei Z
中科院分区：
文献类型：
--
作者：
Braynen J;Yang Y;Wei F;Cao G;Shi G;Tian B;Zhang X;Jia H;Wei X;Wei Z
多倍体是植物进化的一个基本过程。了解多倍体对植物繁殖的影响对多倍体育种至关重要。在本研究中，我们的细胞学分析首次表明，一个整体的减数分裂过程中明显扭曲的合成多倍体芜菁相比，其二倍体祖先。为了在分子水平上阐明这种不规则减数分裂的遗传基础，进一步使用比较RNA-seq分析来研究同源四倍体和二倍体B之间在减数分裂时鉴定的发育花芽的差异遗传调控。拉帕。与二倍体植株相比，同源四倍体B的花芽中共有40，927个表达基因，其中4，601个差异表达基因（DEG）被鉴定出来。其中288个DEG参与了减数分裂。值得注意的是，DMC 1被鉴定为参与DNA双链断裂（DSB）的同源染色体间依赖性修复的一个先前已知的减数分裂特异性基因，在同源四倍体B中显著下调。rapa，这可能有助于减数分裂I期间的异常进展。虽然某些DEG与RNA解旋酶，细胞周期，体细胞DNA修复基因组复制后上调，与减数分裂DSB修复相关的基因显着下调。此外，在两个B中通过实时定量PCR分析证实了通过RNA-seq分析随机选择的DEG的表达。rapa和拟南芥。我们的研究结果首先说明了多倍体在细胞学和转录组学水平上对整个减数分裂过程的不利影响，并允许全面了解二倍体和多倍体B之间减数分裂时花芽转录组的均匀性和差异。拉帕也是。
Polyploidy is a fundamental process in plant evolution. Understanding the polyploidy-associated effects on plant reproduction is essential for polyploid breeding program. In the present study, our cytological analysis firstly demonstrated that an overall course of meiosis was apparently distorted in the synthetic polyploid Brassica rapa in comparison with its diploid progenitor. To elucidate genetic basis of this irregular meiosis at a molecular level, the comparative RNA-seq analysis was further used to investigate differential genetic regulation of developing floral buds identified at meiosis between autotetraploid and diploid B. rapa. In total, compared to its diploid counterparts, among all 40,927 expressed genes revealed, 4,601 differentially expressed genes (DEGs) were identified in the floral buds of autotetraploid B. rapa, among which 288 DEGs annotated were involved in meiosis. Notably, DMC1 identified as one previously known meiosis-specific gene involved in inter-homologous chromosome dependent repair of DNA double stranded breaks (DSBs), was significantly down-regulated in autotetraploid B. rapa, which presumably contributed to abnormal progression during meiosis I. Although certain DEGs associated with RNA helicase, cell cycling, and somatic DNA repair were up-regulated after genome duplication, genes associated with meiotic DSB repair were significantly down-regulated. Furthermore, the expression of randomly selected DEGs by RNA-seq analysis was confirmed by quantitative real-time PCR analysis in both B. rapa and Arabidopsis thaliana. Our results firstly account for adverse effects of polyploidy on an entire course of meiosis at both cytological and transcriptomic levels, and allow for a comprehensive understanding of the uniformity and differences in the transcriptome of floral buds at meiosis between diploid and polyploid B. rapa as well.