TFF3 and EGF induce different migration patterns of intestinal epithelial cells in vitro and trigger increased internalization of E-cadherin
TFF3 and EGF induce different migration patterns of intestinal epithelial cells in vitro and trigger increased internalization of E-cadherin
复制标题
DOI：
10.1159/000107519
复制
发表时间：
2007-01-01
影响因子：
--
通讯作者：
Hoffmann, Werner
中科院分区：
文献类型：
--
作者：
Duerer, Uwe;Hartig, Roland;Hoffmann, Werner
背景/目的：TFF3（TFF（三叶因子家族）肽的成员）和表皮生长因子（EGF）积极支持粘膜屏障的修复，特别是在恢复过程中。本研究的目的是比较 TFF3 和 EGF 的运动原作用。方法：借助延时视频显微镜、形态测定法和免疫细胞化学（包括共聚焦激光扫描显微镜），在体外恢复模型（划伤试验）中表征重组人 TFF3（二聚体形式）和 EGF 对 IEC18 细胞迁移的影响。结果：TFF3 和 EGF 处理的细胞通过不同的迁移模式重新填充受伤区域； TFF3处理导致形成仅具有几个间隙的连续的迁移细胞片。相比之下，经EGF处理的细胞形成了一个由迁移细胞组成的网络（通常具有成纤维细胞样形态），具有大量间隙和仅有点状接触。 TFF3 和 EGF 处理还改变了 E-钙粘蛋白的定位，表明 E-钙粘蛋白的内吞再循环和/或降解。结论：与 EGF 相比，TFF3 增强了集体细胞迁移，确保了重新填充区域的精确覆盖，避免了间隙。版权所有 (c) 2007 S. Karger AG，巴塞尔。
Background/Aims: TFF3, a member of the TFF (trefoil factor family) peptides, and epidermal growth factor (EGF) actively support the repair of mucosal barriers, particularly during restitution. The aim of this study was to compare the motogenic effects of TFF3 and EGF. Methods: The influence of recombinant human TFF3 ( dimeric form) and EGF on the migration of IEC18 cells was characterized in an in vitro restitution model ( scratch wound assay) with the help of time-lapse video microscopy, morphometry, and immunocytochemistry including confocal laser scanning microscopy. Results: TFF3- and EGF-treated cells re-populated the wounded area via different migration patterns; TFF3 treatment resulted in the formation of continuous sheets of migrating cells with only a few gaps. In contrast, EGF-treated cells formed a network of migrating cells (often with a fibroblast-like morphology) with numerous gaps and only punctual contacts. TFF3 and EGF treatment also changed the localization of E-cadherin indicating endocytotic recycling and/or degradation of E-cadherin. Conclusion: TFF3, in contrast to EGF, enhanced a collective cell migration ensuring a precise coverage of the re-populated area avoiding gaps. Copyright (c) 2007 S. Karger AG, Basel.