Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda
Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda
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DOI：
10.1016/j.jviromet.2021.114075
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发表时间：
2021-02-05
影响因子：
3.1
通讯作者：
Malmberg, Maja
中科院分区：
文献类型：
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作者：
Balinandi, Stephen;von Bromssen, Claudia;Malmberg, Maja
背景资料：克里米亚-刚果出血热（CCHF）是由克里米亚-刚果出血热病毒（CCHFV）引起的一种严重的蜱传病毒性出血性疾病，在非洲、欧洲和亚洲的许多地区构成严重的公共卫生挑战。我们使用内部和商业上可用的（IDVet）ELISA检测了来自五个地区的500份牛血清样品的CCHFV抗体，免疫荧光法（IFA）和实时荧光定量PCR（RT-PCR）。（73.8%的女性）进行了分析; CCHFV血清阳性率分别为12.6%（n = 63）和75.0%（n = 375）与内部和IDVet ELISA。血清阳性与地理位置、年龄增长、女性和蜱虫负荷较高有关。IFA检测37例中24例（64.8%）血清学阳性，RT-PCR检测均为阴性。IFA结果与IDVet相比更具可比性（kappa（系数）= 0.88，p = 0.001）。
Background: Crimean-Congo Hemorrhagic Fever (CCHF) is a severe tick-borne viral hemorrhagic disease caused by Crimean-Congo Hemorrhagic Fever Virus (CCHFV) that poses serious public health challenges in many parts of Africa, Europe and Asia.Methods: We examined 500 cattle sera samples from five districts for CCHFV antibodies using in-house and commercially available (IDVet) ELISA, Immunofluorescent assay (IFA) and Real-time polymerase chain reaction (RT-PCR).Results: 500 cattle (73.8 % females) were analyzed; CCHFV seropositivity was 12.6 % (n = 63) and 75.0 % (n = 375) with the in-house and IDVet ELISAs, respectively. Seropositivity was associated with geographical location, increasing age, being female, and having a higher tick burden. Twenty four out of the 37 (64.8 %) were seropositive for CCHFV using IFA and all were negative for virus on RT-PCR. The IFA results were more com-parable to IDVet (kappa(coefficient) = 0.88, p =