Contribution of periodontal pathogens on tongue dorsa analyzed with real-time PCR to oral malodor
Contribution of periodontal pathogens on tongue dorsa analyzed with real-time PCR to oral malodor
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DOI：
10.1016/j.micinf.2004.05.021
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发表时间：
2004-10-01
影响因子：
5.8
通讯作者：
Shizukuishi, S
中科院分区：
文献类型：
--
作者：
Tanaka, M;Yamamoto, Y;Shizukuishi, S
口腔异味被认为主要来源于舌头的微生物群。然而，口腔异味与舌头微生物群之间的关系尚不清楚。本研究采用实时荧光定量PCR技术对牙周病原菌进行分析，并探讨口腔异味与牙周病原菌牙龈卟啉单胞菌、连翘单宁菌、中普雷沃氏菌、黑化普雷沃氏菌和牙密螺旋体的关系。受试者包括29名口腔异味患者和10名健康无口腔异味者。用感官试验评价口腔异味，用气相色谱法测定挥发性硫化合物（VSC）含量。采用LightCycler （TM）系统对舌生物膜样品中的厌氧菌进行实时荧光定量PCR检测；此外，细菌比例作为定量参数。5种厌氧菌中，只有连翘在恶臭受试者中的比例高于健康对照组的相应值。与硫化氢浓度有较强的相关性。牙龈假单胞菌和黑根假单胞菌的比例也与甲基硫醇浓度有很强的相关性。5种厌氧菌总比例与总VSC水平的相关系数（r = 0.88）大于细菌比例与感官评分的相关系数（r = 0.29）。当使用五种牙周病原体的比例作为自变量进行线性回归分析时，这些自变量的解释能力显示VSC总水平为81%，感官评分为16%。这些结果表明，舌背上的这五种牙周病原体可能在VSC的产生中起重要作用。(c) 2004年Elsevier SAS。版权所有。
Oral malodor is considered to originate primarily from tongue microbiota populations. However, the relationship between oral malodor and tongue microbiota remains unclear. In this study, tongue periodontal pathogens were analyzed via real-time PCR, and the association between oral malodor and tongue periodontal pathogens, including Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, Prevotella nigrescens and Treponema denticola, was examined. The subject population consisted of 29 individuals with and 10 healthy persons without oral malodor. Oral malodor was assessed by organoleptic test and volatile sulfur compound (VSC) levels as measured by gas chromatography. Real-time PCR was conducted for anaerobes in tongue biofilm samples employing a LightCycler (TM) system; furthermore, bacterial proportion served as a quantitative parameter. Among the five anaerobes, only T forsythia displayed higher proportions in malodor subjects than corresponding values in healthy controls. Proportions of P. intermedia and R nigrescens correlated strongly with hydrogen sulfide concentration. Proportions of P. gingivalis and P. nigrescens also exhibited strong correlation with methyl mercaptan concentration. The correlation coefficient between the proportion of the total of the five anaerobes and total VSC level (r = 0.88) was greater than that between bacterial proportion and organoleptic score (r = 0.29). When a linear regression analysis was performed utilizing the proportion of each of the five periodontal pathogens as an independent variable, the explanatory power of these independent variables revealed 81% for total VSC level and 16% for organoleptic score. These results suggest that these five periodontal pathogens on tongue dorsa may contribute greatly to VSC production. (c) 2004 Elsevier SAS. All rights reserved.