Expression of fascin-1, the gene encoding the actin-binding protein fascin-1, during mouse embryogenesis
Expression of fascin-1, the gene encoding the actin-binding protein fascin-1, during mouse embryogenesis
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DOI：
10.1016/j.modgep.2004.04.012
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发表时间：
2004-10-01
影响因子：
1.2
通讯作者：
Adams, JC
中科院分区：
文献类型：
--
作者：
De Arcangelis, A;Georges-Labouesse, E;Adams, JC
Fascin-1是一种肌动蛋白捆绑蛋白，在细胞粘附、相互作用和运动中有助于细胞突起和微丝的结构和功能。Fascin-1已在培养细胞中和通过生物物理方法进行了研究，但对其在脊椎动物发育中的分布和功能知之甚少。作为理解肌成束蛋白-1在胚胎发生中的作用的第一步，我们通过对胚胎日（E）8.0-E16.5的整装和切片小鼠胚胎进行原位杂交来表征肌成束蛋白-1的表达模式。Fascin-1在整个胚胎中广泛表达，发育中的神经系统和间充质组织是表达的主要部位。在脑的不同区域、脊髓和视网膜中观察到强信号，颅和背根神经节（DRG）呈强阳性。这种神经表达在整个发育过程中保持强烈。Fascin-1也存在于发育中的体节中。在鳃弓和肢芽间充质中高表达。在后期阶段，fascin-1在面部的不同肌肉、身体的骨骼肌和几个器官的平滑肌层中表达。肢体肌腱呈强阳性。心室内有弱表达。这些结果表明，肌成束蛋白-1主要表达在胚胎发育过程中的神经和间充质衍生物。(C)2004 Elsevier B. V.保留所有权利。
Fascin-1 is an actin-bundling protein that contributes to the architecture and function of cell protrusions and microfilaments in cell adhesion, interactions and motility. Fascin-1 has been studied in cultured cells and by biophysical methods, but little is known about its distribution and functions in vertebrate development. As a first step to understanding the role of fascin-1 in embryogenesis, we have characterised the expression pattern of fascin-1 by in situ hybridisation on whole-mount and sectioned mouse embryos from embryonic day (E)8.0-E16.5. Fascin-1 was widely expressed throughout the embryo and the developing nervous system and mesenchymal tissues represented major sites of expression. Intense signals were observed in different regions of the brain, in the spinal cord and retina, and the cranial and dorsal root ganglia (DRG) appeared strongly positive. This neural expression remained strong throughout development. Fascin-1 was also present in the developing somites. High expression was detected in branchial arches and limb bud mesenchyme. At later stages, fascin-1 was expressed in different muscles of the face, skeletal muscles of the body, and in smooth muscle layers of several organs. Limb tendons appeared strongly positive. There was weak expression in heart ventricles. These results show that fascin-1 is principally expressed in neural and mesenchymal derivatives during embryonic development. (C) 2004 Elsevier B.V. All rights reserved.