The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors
The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors
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DOI：
10.1186/s13071-020-3887-8
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发表时间：
2020-01-15
影响因子：
3.2
通讯作者：
Ferguson, Heather M.
中科院分区：
文献类型：
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作者：
Ortega-Lopez, Leonardo D.;Pondeville, Emilie;Ferguson, Heather M.
伊蚊媒介昆虫学监测主要依赖于对幼虫、蛹和非寄主成虫的监测，这些与人类疾病发病率的相关性很差。接触蚊媒疾病可以更直接地估计使用人类登陆渔获物（HLC），虽然这种方法不推荐用于伊蚊传播的虫媒病毒。我们评估了一种以前用疟疾媒介测试过的新方法，即蚊子电击诱捕器（MET），作为测量伊蚊在人身上着陆率的无风险替代方法。目的是（一）比较MET的BG-哨兵（BGS）陷阱金标准方法的主机寻求伊蚊媒介的采样;和（ii）描述伊蚊媒介的昼夜活动及其与小气候条件的关联。方法该研究于2017年5月在Quininde（厄瓜多尔）进行了12天。在4栋房屋的住宅周边区域设置了苔藓采样站。在采样的每一天，每个房子被分配一个MET或BGS陷阱，这是在拉丁方设计的四个房子之间每天轮换。在每个采样站7：00-19：00之间每小时记录一次苔藓虫丰度和小气候条件，以评估病媒丰度、诱捕方法和环境条件之间的变化。对所有埃及伊蚊雌性进行了寨卡（ZIKV）、登革热（DENV）和基孔肯雅（CHIKV）病毒的检测。结果Ae.埃及女性被发现在MET比在BGS收集，虽然没有统计学上的显着差异，平均Ae。两种诱捕方法均显示雌性埃及伊蚊。埃及人有双峰模式的主机寻找，是在清晨和傍晚时分最高。平均Ae。埃及伊蚊日丰度与日温度呈负相关。在通过任一诱捕方法捕获的任何伊蚊中均未检测到ZIKV、DENV或CHIKV感染。结论我们得出的结论是，MET的表现至少与BGS标准一样好，并提供了直接测量人均人类叮咬率的额外优势。如果在未来的研究中能够证实在MET采集的伊蚊中检测到虫媒病毒，该监测方法将为人类虫媒病毒暴露风险的监测和预测提供有价值的工具。
Background Entomological monitoring of Aedes vectors has largely relied on surveillance of larvae, pupae and non-host-seeking adults, which have been poorly correlated with human disease incidence. Exposure to mosquito-borne diseases can be more directly estimated using human landing catches (HLC), although this method is not recommended for Aedes-borne arboviruses. We evaluated a new method previously tested with malaria vectors, the mosquito electrocuting trap (MET) as an exposure-free alternative for measuring landing rates of Aedes mosquitoes on people. Aims were to (i) compare the MET to the BG-sentinel (BGS) trap gold standard approach for sampling host-seeking Aedes vectors; and (ii) characterize the diel activity of Aedes vectors and their association with microclimatic conditions. Methods The study was conducted over 12 days in Quininde (Ecuador) in May 2017. Mosquito sampling stations were set up in the peridomestic area of four houses. On each day of sampling, each house was allocated either a MET or a BGS trap, which were rotated amongst the four houses daily in a Latin square design. Mosquito abundance and microclimatic conditions were recorded hourly at each sampling station between 7:00-19:00 h to assess variation between vector abundance, trapping methods, and environmental conditions. All Aedes aegypti females were tested for the presence of Zika (ZIKV), dengue (DENV) and chikungunya (CHIKV) viruses. Results A higher number of Ae. aegypti females were found in MET than in BGS collections, although no statistically significant differences in mean Ae. aegypti abundance between trapping methods were found. Both trapping methods indicated female Ae. aegypti had bimodal patterns of host-seeking, being highest during early morning and late afternoon hours. Mean Ae. aegypti daily abundance was negatively associated with daily temperature. No infection by ZIKV, DENV or CHIKV was detected in any Aedes mosquitoes caught by either trapping method. Conclusion We conclude the MET performs at least as well as the BGS standard and offers the additional advantage of direct measurement of per capita human-biting rates. If detection of arboviruses can be confirmed in MET-collected Aedes in future studies, this surveillance method could provide a valuable tool for surveillance and prediction on human arboviral exposure risk.