Dissecting timing variability in yeast meiosis
Dissecting timing variability in yeast meiosis
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DOI：
10.1016/j.cell.2007.09.044
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发表时间：
2007-11-02
期刊：
影响因子：
64.5
通讯作者：
Ramanathan, Sharad
中科院分区：
文献类型：
--
作者：
Nachman, Iftach;Regev, Aviv;Ramanathan, Sharad
细胞命运变化时间上的细胞间变异性对在波动环境中生长的单细胞生物体种群是有利的。我们研究了酿酒酵母减数分裂过程中的时间变异性，开始于营养饥饿。我们使用延时荧光显微镜来测量单细胞减数分裂事件的时间，发现减数分裂的持续时间在细胞之间是高度不同的。这种变异性集中在饥饿开始和早期减数分裂基因开始之间。细胞周期的可变性和营养史对这种时间可变性的影响很小。相反，减数分裂主调制子Ime1的产量的变化及其随时间的逐渐增加支配着这种变异性，细胞大小效应通过Ime1进行传导。这些结果将表型可变性与转录调控因子的表达动态联系在一起，并为研究时间发育过程提供了一个一般框架。
Cell-to-cell variability in the timing of cell-fate changes can be advantageous for a population of single-celled organisms growing in a fluctuating environment. We study timing variability during meiosis in Saccharomyces cerevisiae, initiated upon nutritional starvation. We use time-lapse fluorescence microscopy to measure the timing of meiotic events in single cells and find that the duration of meiosis is highly variable between cells. This variability is concentrated between the beginning of starvation and the onset of early meiosis genes. Cell-cycle variability and nutritional history have little effect on this timing variability. Rather, variation in the production rate of the meiotic master regulator Ime1 and its gradual increase over time govern this variability, and cell size effects are channeled through Ime1. These results tie phenotypic variability with expression dynamics of a transcriptional regulator and provide a general framework for the study of temporal developmental processes.