SUGGESTED EXPLANATION FOR PARADOXICALLY SLOW GROWTH-RATE OF BASAL-CELL CARCINOMAS THAT CONTAIN NUMEROUS MITOTIC FIGURES
SUGGESTED EXPLANATION FOR PARADOXICALLY SLOW GROWTH-RATE OF BASAL-CELL CARCINOMAS THAT CONTAIN NUMEROUS MITOTIC FIGURES
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DOI：
10.1002/path.1711070107
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发表时间：
1972-01-01
影响因子：
7.3
通讯作者：
SEARLE, J
中科院分区：
文献类型：
--
作者：
KERR, JFR;SEARLE, J
材料和方法通过常规组织学技术对三十个连续手术切除的皮肤基底细胞癌进行了检查。来自四个肿瘤的组织也进行了电子显微镜处理：1 mm3 块在冰冷的 5% 中混合 2 小时。二甲胂酸缓冲戊二醛溶液，用缓冲液洗涤并后固定 90 分钟。冰冷的百分之一。二甲胂酸盐缓冲的四氧化锇溶液。进一步清洗后，这些块被染色了 5%。醋酸双氧铀溶液 30 分钟，乙醇脱水并包埋于 Epon 812 中。1-pm 切片用甲苯胺蓝染色并用
MATERIALS AND METHODSThirty consecutive surgically excised basal-cell carcinomas of skin were examined by conventional histological techniques. Tissue from four of the tumours was also processed for electron microscopy: 1 mm3 blocks were iixed for 2 hr in ice-cold 5 per cent. cacodylatebuffered glutaraldehyde solution, washed with buffer and post-fixed for 90 min. in ice-cold 1 per cent. cacodylate-buffered osmium tetroxide solution. After further washing, the blocks were stained in 5 per cent. uranyl acetate solution for 30 min., dehydrated in ethanol and embedded in Epon 812. 1-pm sections were stained with toluidine blue and studied with a