Extension of neurites on axons is impaired by antibodies against specific neural cell surface glycoproteins.
Extension of neurites on axons is impaired by antibodies against specific neural cell surface glycoproteins.
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DOI：
10.1083/jcb.104.2.355
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发表时间：
1987-02
影响因子：
7.8
通讯作者：
Raper, J A
中科院分区：
文献类型：
--
作者：
Chang, S;Rathjen, F G;Raper, J A
我们开发了一种体外测定法，可测量生长锥在轴突基质上延伸的能力。在存在或不存在针对特定神经细胞表面糖蛋白的单价抗体的情况下比较神经突长度。抗神经细胞粘附分子 NCAM 的抗体的 Fab 片段对轴突基质或层粘连蛋白基质上伸长的神经突长度没有显着影响。针对两种新神经细胞表面抗原（由 mAb G4 和 mAb F11 定义）的多克隆抗体的 Fab 片段可减少轴突基质上伸长的神经突长度，但对层粘连蛋白基质上伸长的神经突长度没有影响。 G4 抗原与小鼠 L1 相关，而 F11 抗原似乎与所有已知的神经细胞表面糖蛋白不同。我们的结果表明 G4 和 F11 抗原有助于促进轴突上生长锥的延伸。
We have developed an in vitro assay which measures the ability of growth cones to extend on an axonal substrate. Neurite lengths were compared in the presence or absence of monovalent antibodies against specific neural cell surface glycoproteins. Fab fragments of antibodies against the neural cell adhesion molecule, NCAM, have an insignificant effect on the lengths of neurites elongating on either an axonal substrate or a laminin substrate. Fab fragments of polyclonal antibodies against two new neural cell surface antigens, defined by mAb G4 and mAb F11, decrease the lengths of neurites elongating on an axonal substrate, but have no effect on the lengths of neurites elongating on a laminin substrate. G4 antigen is related to mouse L1, while F11 antigen appears to be distinct from all known neural cell surface glycoproteins. Our results suggest that the G4 and F11 antigens help to promote the extension of growth cones on axons.