The Activity of Class I, II, III, and IV Alcohol Dehydrogenase (ADH) Isoenzymes and Aldehyde Dehydrogenase (ALDH) in Esophageal Cancer
The Activity of Class I, II, III, and IV Alcohol Dehydrogenase (ADH) Isoenzymes and Aldehyde Dehydrogenase (ALDH) in Esophageal Cancer
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DOI：
10.1007/s10620-008-0422-8
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发表时间：
2009-04-01
影响因子：
3.1
通讯作者：
Szmitkowski, Maciej
中科院分区：
文献类型：
--
作者：
Jelski, Wojciech;Kozlowski, Miroslaw;Szmitkowski, Maciej
背景/目标 乙醇消耗与食道癌风险增加相关。致癌化合物是乙醛，是乙醇代谢的产物。乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）是参与乙醇代谢的主要酶，导致乙醛的产生。本研究将食管癌中 ADH 同工酶和 ALDH 的活性与正常组织中的活性进行了比较。方法采用荧光法测定I类和II类ADH同工酶和ALDH活性。通过光度法测定总ADH活性以及III类和IV类同工酶的活性。样本取自 59 名食管癌患者（27 名腺癌，32 名鳞状细胞癌）。结果癌细胞中ADH总活性和IV类ADH活性显着高于健康组织。其他测试类别的 ADH 在癌症中表现出比在正常细胞中更高的活性。饮酒者和不饮酒者在癌症和健康组织中的酶活性差异并不显着。结论 ADH IV 活性增加可能是加剧癌变的一个因素，因为乙醇形成乙醛的能力增加。
Background/Aims Ethanol consumption is associated with an increased risk of esophageal cancer. The carcinogenic compound is acetaldehyde, the product of ethanol metabolism. Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are the main enzymes involved in ethanol metabolism, which leads to generation of acetaldehyde. In this study the activity of ADH isoenzymes and ALDH in esophageal cancer were compared with the activity in normal tissue. Methods For measurement of the activity of class I and II ADH isoenzymes and ALDH activity fluorimetric methods were employed. Total ADH activity and activity of class III and IV isoenzymes was measured by the photometric method. Samples were taken from 59 esophageal cancer patients (27 adenocarcinoma, 32 squamous cell cancer). Results The total activity of ADH and activity of class IV ADH were significantly higher in cancer cells than in healthy tissues. The other tested classes of ADH showed a tendency toward higher activity in cancer than in normal cells. Differences between the activity of enzymes of drinkers and non-drinkers in both cancer and healthy tissue were not significant. Conclusion Increased ADH IV activity may be a factor intensifying carcinogenesis, because of the increased ability to form acetaldehyde from ethanol.