Vaporized Perfluorocarbon Confers Protection Against Acute Lung Injury by Inhibiting MMP-9 Expression without Protective Effects in Other Organs
Vaporized Perfluorocarbon Confers Protection Against Acute Lung Injury by Inhibiting MMP-9 Expression without Protective Effects in Other Organs
复制标题
汽化全氟化碳通过抑制 MMP-9 表达来预防急性肺损伤，但对其他器官没有保护作用
DOI：
10.1177/147323001204000112
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发表时间：
2012-01-01
影响因子：
1.6
通讯作者：
Xie, L.
中科院分区：
文献类型：
--
作者：
Han, B.;Zhao, X.;Xie, L.
目的：全氟化碳(PFC)是一种治疗肺损伤的药物，本研究探讨了其作用机制及对其他器官的潜在保护作用。方法：32只雌性新西兰大白兔采用人工海水肺灌洗法复制急性肺损伤模型。动物接受常规机械通气(CMV)、CMV下呼气末正压(PEEP)、雾化PFC通气或呼气末正压+雾化PFC通气(PEEP+PFC)。取肺、小肠、肝、肾进行组织病理学观察。检测肺组织中基质金属蛋白酶-9(MMP9)的表达。结果：与CMV组比较，PEEP组、PFC组和PEEP+PFC组肺组织病理损伤明显减轻。各组大鼠肝、肾、小肠组织损伤程度相似。与CMV组比较，PEEP组、PFC组和PEEP+PFC组的MMP9基因表达水平显著降低。结论：雾化PFC呼吸机可明显减轻肺损伤，但对其他脏器无明显保护作用。肺损伤的减轻可能与抑制基质金属蛋白酶-9有关。
OBJECTIVE: Vaporized perfluorocarbon (PFC) is a treatment for lung injury; this study investigated its mode of action and potential protective effects on other organs, which are unclear. METHODS: Acute lung injury was induced by lung lavage with artificial seawater in 32 female New Zealand White rabbits. Animals received either conventional mechanical ventilation (CMV), positive end-expiratory pressure under CMV (PEEP), vaporized PFC ventilation, or positive end-expiratory pressure with vaporized PFC ventilation (PEEP + PFC). Histopathology of the lung, small intestine, liver and kidney were investigated. Matrix metalloproteinase (MMP)-9 mRNA levels in the lung were analysed. RESULTS: Pathological injury of the lung was significantly alleviated in the PEEP, PFC and PEEP + PFC groups compared with the CMV group. Tissue damage in the liver, kidney and small intestine was similar between all groups. MMP-9 mRNA levels in the PEEP, PFC and PEEP + PFC groups were significantly lower than those in the CMV group. CONCLUSIONS: Vaporized PFC ventilation can significantly alleviate lung injury but has no significant protective effect on other organs. Alleviation of lung injury may be associated with MMP-9 inhibition.