Repair of the tRNA-Like CCA sequence in a multipartite positive-strand RNA virus
Repair of the tRNA-Like CCA sequence in a multipartite positive-strand RNA virus
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DOI：
10.1128/jvi.79.3.1417-1427.2005
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发表时间：
2005-02-01
影响因子：
5.4
通讯作者：
Kao, C
中科院分区：
文献类型：
--
作者：
Hema, M;Gopinath, K;Kao, C
正链雀麦花叶病毒 (BMV) RNA 的 3' 部分模仿细胞 tRNA。 tRNA 样序列 (TLS) 3' CCA 中的核苷酸取代或缺失会影响负链起始，除非修复。我们观察到，涉及一种或所有 BMV RNA 中的 CCA 3' 序列的 2 核苷酸缺失仍然允许大麦原生质体中 RNA 以显着水平积累。仅 BMV RNA3 中的 CCA 至 GGA 的改变也允许野生型水平的 RNA 积累。然而，所有三种 BMV RNA 中的替换都严重减少了 RNA 积累，这表明替换与小缺失具有不同的修复要求。此外，具有核苷酸取代的RNA的修复和复制需要野生型BMV RNA1。对突变输入 RNA 的子代病毒 RNA 进行测序的结果表明，RNA1 并未将其序列贡献给突变 RNA。相反，修复的末端是异质的，其中三分之一具有恢复的CCA，而其他末端的序列唯一的共同点是恢复了一个胞苷酸。检查了 BMV RNA1 在增强修复中的作用。
The 3' portions of plus-strand brome mosaic virus (BMV) RNAs mimic cellular tRNAs. Nucleotide substitutions or deletions in the 3' CCA of the tRNA-like sequence (TLS) affect minus-strand initiation unless repaired. We observed that 2-nucleotide deletions involving the CCA 3' sequence in one or all BMV RNAs still allowed RNA accumulation in barley protoplasts at significant levels. Alterations of CCA to GGA in only BMV RNA3 also allowed RNA accumulation at wild-type levels. However, substitutions in all three BMV RNAs severely reduced RNA accumulation, demonstrating that substitutions have different repair requirements than do small deletions. Furthermore, wild-type BMV RNA1 was required for the repair and replication of RNAs with nucleotide substitutions. Results from sequencing of progeny viral RNA from mutant input RNAs demonstrated that RNA1 did not contribute its sequence to the mutant RNAs. Instead, the repaired ends were heterogeneous, with one-third having a restored CCA and others having sequences with the only commonality being the restoration of one cytidylate. The role of BMV RNA1 in increased repair was examined.