A General RNA Motif for Cellular Transfection
A General RNA Motif for Cellular Transfection
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DOI：
10.1038/mt.2011.277
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发表时间：
2012-03-01
影响因子：
12.4
通讯作者：
Levy, Matthew
中科院分区：
文献类型：
--
作者：
Magalhaes, Maria L. B.;Byrom, Michelle;Levy, Matthew
我们开发了一种选择方案来产生识别并直接内化到哺乳动物细胞中的核酸序列，而无需常规递送方法的帮助。为了证明该技术的通用性，针对不同的靶细胞进行了两次具有不同起始池的独立选择。每次选择都会产生一个单一的高功能序列，两者都折叠成一个共同的核心结构。该内化信号可适合用作通用试剂，用于转染到包括原代细胞在内的多种细胞类型中。 2011 年 8 月 31 日收到； 2011 年 11 月 19 日接受； 2012 年 1 月 10 日在线发布。doi:10.1038/mt.2011.277
We have developed a selection scheme to generate nucleic acid sequences that recognize and directly internalize into mammalian cells without the aid of conventional delivery methods. To demonstrate the generality of the technology, two independent selections with different starting pools were performed against distinct target cells. Each selection yielded a single highly functional sequence, both of which folded into a common core structure. This internalization signal can be adapted for use as a general purpose reagent for transfection into a wide variety of cell types including primary cells. Received 31 August 2011; accepted 19 November 2011; published online 10 January 2012. doi:10.1038/mt.2011.277