Sympathoadrenal-dependent sexually dimorphic effect of nonhabituating stress on in vivo neutrophil recruitment in the rat
Sympathoadrenal-dependent sexually dimorphic effect of nonhabituating stress on in vivo neutrophil recruitment in the rat
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DOI：
10.1038/sj.bjp.0706257
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发表时间：
2005-08-01
影响因子：
7.3
通讯作者：
Green, PG
中科院分区：
文献类型：
--
作者：
Barker, LA;Dazin, PF;Green, PG
由于应激既激活交感肾上腺轴又深刻影响炎症和炎症性疾病，其中许多是两性二态的，因此我们测试了应激对中性粒细胞募集的影响（急性炎症反应的主要组成部分）是否具有两性二态。2 .在大鼠气囊模型中，研究了间歇性声音（超过4天）对脂多糖（LPS）诱导的中性粒细胞募集的影响。在最后一次应激暴露后24小时，lps诱导的中性粒细胞募集在雄性大鼠中增强，而在雌性大鼠中没有当在青春期前进行性腺切除术并在成年后进行测试时，应激显著抑制了雄性中lps诱导的中性粒细胞募集的大小，而对性腺切除术的雌性仍然没有影响。在男性中，肾上腺去神经支配后，应激引起的lps诱导的中性粒细胞募集的增加被阻止。由于这些数据表明压力的影响依赖于交感肾上腺轴，我们测试了儿茶酚胺介导压力效应的假设在雄性大鼠中，持续给予β -肾上腺素能受体拮抗剂心得安(4 mg kg(-1) day(-1))，在声应激暴露期间，应激对lps诱导的中性粒细胞募集的影响显著减弱，而给予异丙肾上腺素（10 nmol, i.v）显著增加雄性中性粒细胞募集，其效果在定性和定量上与应激的效果相似。心得安显著增加了非应激雌性大鼠的中性粒细胞募集，而对应激雌性大鼠的中性粒细胞募集无显著影响这些发现表明，应激对中性粒细胞迁移（炎症反应的主要组成部分）的交感肾上腺依赖效应中存在明显的雄性性激素依赖性二态性，并表明交感肾上腺轴通过肾上腺素的释放参与了这种效应。
1 Since stress both activates the sympathoadrenal axis and profoundly affects inflammation and inflammatory diseases, many of which are sexually dimorphic, we tested whether the effect of stress on neutrophil recruitment, a primary component of the acute inflammatory response, is sexually dimorphic. 2 The effect of intermittent sound (over 4 days), a nonhabituating stress, on lipopolysaccharide (LPS)-induced recruitment of neutrophils was evaluated in vivo in the rat air pouch model. At 24 h following the last stress exposure, LPS-induced neutrophil recruitment was enhanced in male rats, but not in females.3 When gonadectomized prepubertally and tested as adults, stress significantly inhibited the magnitude of LPS-induced neutrophil recruitment in males, while it still had no effect in gonadectomized females. In males, following adrenal denervation, the increase in LPS-induced neutrophil recruitment produced by stress was prevented. Since these data suggest that the effect of stress is dependent on the sympathoadrenal axis, we tested the hypothesis that catecholamines mediate the stress effects.4 In male rats, the effect of stress on LPS-induced neutrophil recruitment was significantly attenuated by continuous administration of the beta-adrenergic receptor antagonist, propranolol (4 mg kg(-1) day(-1)), during sound stress exposure, and administration of isoproterenol (10 nmoles, i.v.) significantly increased neutrophil recruitment in males, an effect that was qualitatively and quantitatively similar to the effect of stress. Propranolol significantly increased neutrophil recruitment in nonstressed female rats, but did not significantly affect neutrophil recruitment in stressed females.5 These findings indicate a marked male sex hormone-dependent sexual dimorphism in the sympathoadrenal-depen dent effect of stress on neutrophil migration, a primary component of the inflammatory response, and suggest that the sympathoadrenal axis contributes to this effect via release of epinephrine.