A role for the mevalonate pathway in the induction of subtype cross-reactive immunity to influenza A virus by human gammadelta T lymphocytes.
A role for the mevalonate pathway in the induction of subtype cross-reactive immunity to influenza A virus by human gammadelta T lymphocytes.
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DOI：
10.1016/j.cellimm.2010.04.013
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发表时间：
2010
影响因子：
4.3
通讯作者：
Ennis, Francis A.
中科院分区：
文献类型：
--
作者：
Jameson, Julie M.;Cruz, John;Costanzo, Anne;Terajima, Masanori;Ennis, Francis A.
人外周血中的主要γδ T细胞亚群表达Vγ9δ2 TCR并识别非肽异戊烯基焦磷酸抗原，如异戊基焦磷酸（IPP）。在活化后，γδ T细胞快速分泌类似于经典记忆αβ T细胞的抗病毒细胞因子。在这里，我们研究了来自人PBMC的γδ T淋巴细胞被甲型流感病毒感染激活的能力。流感病毒感染PBMC后，Vγ 9 V δ2 T淋巴细胞迅速上调CD 25和CD 69的表达，并产生IFN-γ。此外，这种识别在流感的各种亚型之间具有交叉反应性，但不与牛痘病毒发生交叉反应。HMG-CoA还原酶抑制剂美伐他汀可有效降低Vγ 9VS 2 T细胞应答，美伐他汀可抑制甲羟戊酸途径和IPP合成。我们的研究结果表明，流感病毒感染通过依赖于甲羟戊酸途径的机制诱导外周血中Vγ9Vδ2 T淋巴细胞的快速活化和功能。
The major γδ T cell subset in the human peripheral blood expresses the Vγ9δ2 TCR and recognizes non-peptidic prenyl pyrophosphate antigens such as isopentylpyrophosphate (IPP). Upon activation the γδ T cells rapidly secrete antiviral cytokines similar to classical memory αβ T cells. Here we have investigated the ability of γδ T lymphocytes from human PBMC to become activated by influenza A virus infection. Vγ9Vδ2 T lymphocytes rapidly upregulate expression of CD25 and CD69 and produce IFN-γ following influenza infection of PBMC. Moreover, the recognition is cross-reactive between various subtypes of influenza, but not with vaccinia virus. Vγ9Vδ2 T cell responses are potently reduced by the HMG-CoA reductase inhibitor mevastatin, which inhibits the mevalonate pathway and IPP synthesis. Our results indicate that influenza virus infection induces the rapid activation and function of Vγ9Vδ2 T lymphocytes in the peripheral blood via a mechanism that depends on the mevalonate pathway.
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影响因子：
20.3
作者：
Thompson, K;Rojas-Navea, J;Rogers, MJ
通讯作者：
Rogers, MJ
影响因子：
6.4
作者：
Qin, Gang;Mao, Huawei;Tu, Wenwei
通讯作者：
Tu, Wenwei
影响因子：
5
作者：
Terajima, M;Van Epps, HL;Ennis, FA
通讯作者：
Ennis, FA
影响因子：
3.7
作者：
Correia DV;d'Orey F;Cardoso BA;Lança T;Grosso AR;deBarros A;Martins LR;Barata JT;Silva-Santos B
通讯作者：
Silva-Santos B
影响因子：
6.2
作者：
Thompson, K;Rogers, MJ
通讯作者：
Rogers, MJ