High-resolution high-speed synchronous epifluorescence imaging of cardiac activation
High-resolution high-speed synchronous epifluorescence imaging of cardiac activation
复制标题
心脏激活的高分辨率高速同步落射荧光成像
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
J. Wikswo
中科院分区：
文献类型：
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作者：
Shien‐Fong Lin;R. A. Abbas;J. Wikswo
发展了一种具有高空间和时间分辨率的光学成像技术，以记录在单相点刺激期间和之后与心脏跨膜电位相关的激光诱导的荧光的部分变化。该技术利用记录事件的重复性，利用同步激光选通机制克服了慢扫描电荷耦合器件摄像机固有的速度限制，在像素分辨率为75μm的单帧中获得了500帧/S的有效帧速率和100×100像素的像素分辨率，并通过箱车平均提高了信噪比。通过对兔心脏静息心肌的刺激，显示了刺激过程中具有明显同时去极化和超极化区域的虚阴极和虚阳极模式。本文所描述的技术为研究可重复动力学提供了一个强有力的工具。
An optical imaging technique with high spatial and temporal resolution was developed to record fractional changes in laser-induced epifluorescence associated with the cardiac transmembrane potential during and after the application of monophasic point stimuli. The technique takes advantage of the repeatability of the recorded events, and uses a synchronized laser strobing mechanism to overcome the speed limitation inherent to slow-scan charge-coupled device cameras, and achieves an effective frame rate of 500 frames/s at a spatial resolution of 100×100 pixels in a single frame with a pixel resolution of 75 μm. The signal-to-noise ratio can be improved with boxcar averaging. Patterns of virtual cathode and anode with distinctive regions of simultaneous depolarization and hyperpolarization during stimulation are demonstrated with stimuli applied to the resting myocardium of an isolated rabbit heart. The technique described in this article provides a powerful tool for investigating repeatable dynamics in the...