Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape ( Brassica napus )
Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape ( Brassica napus )
复制标题
病原体生活方式决定油菜（甘蓝型油菜）定量抗病位点的宿主遗传特征
DOI：
10.1101/2023.08.02.551671
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Jacott C
中科院分区：
文献类型：
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作者：
Jacott C
关键信息利用关联转录组学，我们的研究鉴定了作物中对四种不同真菌病原体具有抗性的基因，强调了多病原体抗性的关键遗传决定因素。摘要作物受到多种病原体的影响，但很少对这些病原体进行并行研究，以确定控制疾病的常见和独特的遗传因素。广谱定量抗病性（QDR）对于作物育种来说是理想的，因为它赋予对多种病原体物种的抗性。在这里，我们使用关联转录组学 (AT) 来识别与甘蓝型油菜对四种对比真菌病原体的组成性 QDR 相关的候选基因位点：Alternaria brasicicola、Botrytis cinerea、Pyrenopeziza brasicae 和 Verticillium longisporum。我们没有识别出与对具有对比生活方式的真菌病原体的广谱 QDR 相关的任何共享位点。相反，我们观察到 QDR 依赖于病原体的生活方式——半生物营养型和坏死营养型病原体具有不同的 QDR 反应和相关基因座，包括一些与早期免疫相关的基因座。此外，我们还发现了与 V 抗性相关的基因组缺失。长孢子虫和潜在的广谱 QDR。这是 AT 首次同时用于多个病理系统，以鉴定与广谱 QDR 相关的宿主基因位点。我们强调广谱 QDR 的组成型表达候选基因座，对作为作物育种候选的其他病原体研究的敏感性没有拮抗作用。总之，这项研究代表了我们对广谱 QDR inB 的理解的进步。 napusand 是科学界的重要资源。
Key messageUsing associative transcriptomics, our study identifies genes conferring resistance to four diverse fungal pathogens in crops, emphasizing key genetic determinants of multi-pathogen resistance.AbstractCrops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use associative transcriptomics (AT) to identify candidate gene loci associated withBrassica napusconstitutive QDR to four contrasting fungal pathogens:Alternaria brassicicola,Botrytis cinerea,Pyrenopeziza brassicae,andVerticillium longisporum.We did not identify any shared loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen—hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance toV. longisporumand potentially broad-spectrum QDR. This is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. We highlight constitutive expressed candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. In conclusion, this study represents an advancement in our understanding of broad-spectrum QDR inB. napusand is a significant resource for the scientific community.