Opposing actions of cGMP and calcium on the conductance of the F0 subunit c pore
Opposing actions of cGMP and calcium on the conductance of the F0 subunit c pore
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DOI：
10.1006/bbrc.2000.3231
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发表时间：
2000-08-11
影响因子：
3.1
通讯作者：
Guidotti, G
中科院分区：
文献类型：
--
作者：
McGeoch, JEM;McGeoch, MW;Guidotti, G
ATP合酶的亚基c可以从神经元质膜和线粒体内膜中纯化。在线粒体内膜中，疏水的75个氨基酸的蛋白质是F-1 F-0 ATP合酶复合物的一个组分，但在前者中，它单独作为能够产生自发电振荡的孔。纯哺乳动物亚基c在脂质双层中重构并电压钳位时，产生电压敏感孔，其传导由钙调节的阳离子电流。在此发现电流被cGMP激活，K-M范围为14 nM至19 μ M，取决于钙和温度。它被许多配体敏感地抑制，钙的K-I范围从100 nM到100 μ M，取决于cGMP和温度。DCCD以100 nM的K-app抑制。多胺尼古丁在84 nM时抑制，孔具有允许其以受控方式通过细胞膜递送钠或钙同时保持膜极化的性质。(C)北京大学出版社.
Subunit c of ATP synthase can be purified from neuronal plasma membrane and from the inner mitochondrial membrane, In the latter location the hydrophobic 75 amino acid protein is one component of the F-1 F-0 ATP synthase complex but in the former it is alone as a pore that is capable of generating spontaneous electrical oscillations. Pure mammalian subunit c when reconstituted in lipid bilayers and voltage clamped, yields a voltage sensitive pore that conducts a cation current regulated by calcium. The current is here found to be activated by cGMP with a K-M ranging from 14 nM to 19 mu M depending on calcium and temperature. It is sensitively inhibited by a number of ligands, The K-I for calcium ranges from 100 nM to 100 mu M depending on cGMP and temperature. DCCD inhibits with a K-app of 100 nM. The polyamine nicotine inhibits at 84 nM, The pore has properties that would allow it to deliver sodium or calcium through the cell membrane in a controlled manner while maintaining membrane polarization. (C) 2000 Academic Press.