Initiation of satellite cell replication in bupivacaine-induced myonecrosis
Initiation of satellite cell replication in bupivacaine-induced myonecrosis
复制标题
布比卡因诱导的肌坏死中卫星细胞复制的启动
DOI：
10.1007/bf00293401
复制
发表时间：
2004
影响因子：
12.7
通讯作者：
I. Nonaka
中科院分区：
文献类型：
--
作者：
Y. Saito;I. Nonaka
为了确定卫星细胞在肌肉再生中如何以及何时被刺激复制，在布比卡因诱导的肌坏死后按时间顺序检查了大鼠比目鱼肌。布比卡因处理后25小时，当巨噬细胞已经侵入坏死纤维的肌浆时，观察到溴脱氧尿苷和结蛋白阳性单核细胞，表明卫星细胞复制开始。这些发现表明，肌肉再生早在巨噬细胞开始清除坏死物质时就开始了。增殖的成肌细胞数量增加，在肌坏死后49小时达到最大值，并在成肌细胞相互融合形成肌管后3天数量减少。布比卡因诱导的肌坏死后卫星细胞增殖几乎与挤压伤同时开始，并且早于使用完整完整或切碎的肌肉碎片进行肌肉移植后的卫星细胞增殖。更早开始和更快速的再生过程可能是由于布比卡因引起的肌坏死中完整的卫星细胞、血管和周围神经的保存所致。
To determine how and when the satellite cells are stimulated to replicate in muscle regeneration, the rat soleus muscle was examined chronologically after bupivacaine-induced myonecrosis. Bromodeoxyuridine and desmin-positive mononuclear cells, indicating the start of satellite cell replication, were seen 25 h after bupivacaine treatment when macrophages had already invaded the sarcoplasm of necrotic fiber. These findings suggest that muscle regeneration starts as early as the time at which macrophages begin to scavenge necrotic material. Proliferating myoblasts increased in number, reaching a maximum at 49 h after myonecrosis, and decreased in number 3 days after the myoblasts fused with each other to form myotubes. The satellite cell proliferation after bupivacaine-induced myonecrosis began at almost the same time as in crush injury, and earlier than after muscle transplantation using whole intact or minced muscle fragments. The earlier begining and more rapid regenerating process probably resulted from the preservation of intact satellite cells, blood vessels and peripheral nerves in the bupivacaine-induced myonecrosis.