Determination of ciprofloxacin with functionalized cadmium sulfide nanoparticles as a fluorescence probe.
Determination of ciprofloxacin with functionalized cadmium sulfide nanoparticles as a fluorescence probe.
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DOI：
10.1016/j.saa.2008.03.024
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
Dan Li;Zheng-yu Yan;Wei-qing Cheng
中科院分区：
文献类型：
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作者：
Dan Li;Zheng-yu Yan;Wei-qing Cheng
基于功能化纳米CdS与环丙沙星相互作用产生的荧光增强信号的测量，提出了一种灵敏度在微克级的环丙沙星的新测定方法。以硫代乙酰胺（TAA）和硝酸镉（Cd（NO3）2）为原料，在碱性溶液中合成了CdS纳米粒子。在pH7.4时，随着环丙沙星浓度的增加，功能化纳米CdS的荧光信号显著增强。环丙沙星浓度在（1.25-8.75）×10− 4 mgmL −1（（3.77-26.4）×10− 4 mmolL −1）或（8.75-1200）×10 − 4 mgmL −1（（26.4-3625）×10− 4 mmolL −1）范围内与荧光增强强度呈线性关系。检测限为7.64×10− 6 mgmL −1（2.31×10− 5 mmolL −1）。在此基础上，建立了一种不需分离杂质直接定量测定人血清样品中环丙沙星的新方法。本法测定人血清中环丙沙星的含量，回收率为95-105%，相对标准偏差（R.S.D.）1.5- 2.5%。该方法灵敏、快速、简便，对大多数干扰物质具有良好的耐受性。
A novel assay of ciprofloxacin with a sensitivity at the microgram level is proposed based on the measurement of enhanced fluorescence intensity signals resulting from the interaction of functionalized nano-CdS with ciprofloxacin. The CdS nanoparticles was synthesized by thioacetamide (TAA) and cadmium nitrate (Cd(NO3)2) in the alkaline solution. At pH 7.4, the fluorescence signals of functionalized nano-CdS were greatly enhanced by ciprofloxacin with the increase concentration of ciprofloxacin. Linear relationship can be established between the enhanced fluorescence intensity and ciprofloxacin concentration in the range of (1.25–8.75)×10−4mgmL−1((3.77–26.4)×10−4mmolL−1) or (8.75–1200)×10−4mgmL−1((26.4–3625)×10−4mmolL−1). The limit of detection is 7.64×10−6mgmL−1(2.31×10−5mmolL−1). Based on this, a new direct quantitative determination method for ciprofloxacin in human serum samples without separation of foreign substances was established. The contents of ciprofloxacin in human serum samples were determined with recovery of 95–105% and relative standard deviation (R.S.D.) of 1.5–2.5%. This method was proved to be very sensitive, rapid, simple and tolerance of most interfering substances.