P27X-III AND P21X-III PROTEINS ENCODED BY THE PX SEQUENCE OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I
P27X-III AND P21X-III PROTEINS ENCODED BY THE PX SEQUENCE OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I
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DOI：
10.1073/pnas.82.24.8359
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发表时间：
1985-12-01
影响因子：
11.1
通讯作者：
YOSHIDA, M
中科院分区：
文献类型：
--
作者：
KIYOKAWA, T;SEIKI, M;YOSHIDA, M
人T细胞白血病病毒I型（HTLV-1）是成人T细胞白血病的病原体，具有独特的序列pX，其包含四个可能的开放阅读框架I-IV。p40 x先前被鉴定为框架IV（x-lor）的基因产物，并被认为介导病毒长末端重复序列的转录反式激活。我们已经确定了两个pX基因产物，p27 x-III和p21 x-III，编码的框架II，其中大部分重叠框架IV。用兔抗血清检测这些蛋白质，所述兔抗血清针对从框架III的3“末端预测的合成肽。p27 x-III在培养的细胞中被磷酸化，并且磷蛋白（pp 27 x-III）定位于细胞核中;一些pp 27 x-III与细胞核组分紧密结合。在许多表达其他病毒抗原的细胞系中检测到p27 x-III，包括先前报道仅表达p40 x作为病毒蛋白的细胞系。p27 x-III和p21 x-III的功能尚不清楚，但pp 27 x-III与核组分的紧密结合表明其与病毒基因表达的调节有关。
Human T-cell leukemia virus type I (HTLV-I) is an etiological agent of adult T-cell leukemia and has a unique sequence, pX, that contains four possible open reading frames, I-IV. p40x was previously identified as the gene product of frame IV (x-lor) and was suggested to mediate transcriptional trans-activation of the viral long terminal repeats. We have identified two pX gene products, p27x-III and p21x-III, encoded by frame II, which mostly overlapped frame IV. These proteins were detected with rabbit antiserum against the synthetic peptide predicted from the 3'' end of frame III. p27x-III is phosphorylated in cultured cells, and the phosphoprotein (pp27x-III) is localized in nuclei; some pp27x-III was tightly bound to nuclear components. p27x-III was detected in a number of cell lines that express other viral antigens, including a cell line previously reported to express only p40x as a viral protein. The function(s) of p27x-III and p21x-III is not known, but the tight binding of pp27x-III to nuclear components suggests that it is associated with regulation of viral gene expression.