Diode probes for spatiotemporal optical control of multiple neurons in freely moving animals
Diode probes for spatiotemporal optical control of multiple neurons in freely moving animals
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DOI：
10.1152/jn.00153.2012
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发表时间：
2012-07-01
影响因子：
2.5
通讯作者：
Buzsaki, Gyoergy
中科院分区：
文献类型：
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作者：
Stark, Eran;Koos, Tibor;Buzsaki, Gyoergy
Stark E、Koos T、Buzsaki G.自由运动动物多神经元时空光学控制的二极管探针。J Neurophysiol 108：349-363，2012.首次发表于2012年4月11日; doi：10.1152/jn.00153.2012.-具有高时间精度的神经元控制是可能的光遗传学，但目前可用的方法不能独立地控制自由移动动物大脑中的多个位置。在这里，我们描述了一种二极管探针系统，该系统允许对多个部位的神经元活动进行实时和特定位置的控制。在任意时空模式中操纵神经元活动是通过光电阵列来实现的，该光电阵列通过将多个二极管-纤维组件连接到高密度硅探针或四极丝来制造，并植入表达光响应视蛋白的动物的大脑中。每个二极管都可以单独控制，允许在任何时间配置中对神经元激活器和沉默器进行局部光刺激，并同时记录受刺激的神经元。由于与动物的唯一连接是通过高度柔性的电线电缆，因此允许在完整的大脑中进行电路监测和多点扰动。该系统产生独特神经活动模式的能力有助于以闭环方式对神经回路进行多点操作，并为解决新问题打开了大门。
Stark E, Koos T, Buzsaki G. Diode probes for spatiotemporal optical control of multiple neurons in freely moving animals. J Neurophysiol 108: 349-363, 2012. First published April 11, 2012; doi:10.1152/jn.00153.2012.-Neuronal control with high temporal precision is possible with optogenetics, yet currently available methods do not enable to control independently multiple locations in the brains of freely moving animals. Here, we describe a diode-probe system that allows real-time and location-specific control of neuronal activity at multiple sites. Manipulation of neuronal activity in arbitrary spatiotemporal patterns is achieved by means of an optoelectronic array, manufactured by attaching multiple diode-fiber assemblies to high-density silicon probes or wire tetrodes and implanted into the brains of animals that are expressing light-responsive opsins. Each diode can be controlled separately, allowing localized light stimulation of neuronal activators and silencers in any temporal configuration and concurrent recording of the stimulated neurons. Because the only connections to the animals are via a highly flexible wire cable, unimpeded behavior is allowed for circuit monitoring and multisite perturbations in the intact brain. The capacity of the system to generate unique neural activity patterns facilitates multisite manipulation of neural circuits in a closed-loop manner and opens the door to addressing novel questions.