Effect of negative pressure on growth, secretion and biofilm formation of Staphylococcus aureus
Effect of negative pressure on growth, secretion and biofilm formation of Staphylococcus aureus
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负压对金黄色葡萄球菌生长、分泌及生物膜形成的影响
DOI：
10.1007/s10482-015-0545-9
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发表时间：
2015-10-01
影响因子：
2.6
通讯作者：
Tang, Peifu
中科院分区：
文献类型：
--
作者：
Li, Tongtong;Wang, Guoqi;Tang, Peifu
负压伤口治疗（NPWT）作为一种有效的物理治疗方法，在污染伤口的管理中越来越受欢迎，尽管其对伤口中细菌的影响尚不清楚。在这项研究中，我们试图探索负压条件对伤口感染中最常见的病原体金黄色葡萄球菌的影响。S.金黄色葡萄球菌在Luria-Bertani培养基中于-125 mmHg的亚大气压下培养24 h，以在环境压力下生长的细菌作为对照。负压的应用被发现减缓了生长速率，抑制了生物膜的形成。金黄色葡萄球菌，这是通过静态生物膜测定证实。此外，毒力因子和生物膜组分的总量也有所减少，包括α-溶血素、胞外粘附蛋白、多糖细胞间粘附素和胞外DNA。通过定量RT-PCR分析，我们还发现了与伤口感染和细菌生物膜相关的毒力和调控基因的转录的显著抑制。这些结果表明，负压可以抑制S.金黄色。局部负压条件（如NPWT）可能是伤口护理领域的潜在抗病毒和抗粘膜策略。
Negative pressure wound therapy (NPWT) has gained popularity in the management of contaminated wounds as an effective physical therapy, although its influence on the bacteria in the wounds remains unclear. In this study, we attempted to explore the effect of negative pressure conditions on Staphylococcus aureus, the most frequently isolated pathogen during wound infection. S. aureus was cultured in Luria-Bertani medium at subatmospheric pressure of -125 mmHg for 24 h, with the bacteria grown at ambient pressure as the control. The application of negative pressure was found to slow down the growth rate and inhibit biofilm development of S. aureus, which was confirmed by static biofilm assays. Furthermore, decreases in the total amount of virulence factors and biofilm components were observed, including α-hemolysin, extracellular adherence protein, polysaccharide intercellular adhesin and extracellular DNA. With quantitative RT-PCR analysis, we also revealed a significant inhibition in the transcription of virulence and regulatory genes related to wound infections and bacterial biofilms. Together, these findings indicated that negative pressure could inhibit the growth, virulence and biofilm formation of S. aureus. A topical subatmospheric pressure condition, such as NPWT, may be a potential antivirulence and antibiofilm strategy in the field of wound care.