Dynamic filtering by two-dimensional 1H NMR with application to phage lambda repressor.
Dynamic filtering by two-dimensional 1H NMR with application to phage lambda repressor.
复制标题
通过二维 1H NMR 动态过滤并应用于噬菌体 lambda 阻遏物。
DOI：
10.1073/pnas.81.19.6019
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
States,DJ
中科院分区：
文献类型：
--
作者：
Weiss,MA;Eliason,JL;States,DJ
蛋白质的柔性区域在催化、配体结合和大分子相互作用中发挥重要作用。由于其增强的灵敏度，运动窄化，二维耦合常数J相关的1H NMR可以用来观察这些区域选择性。动态滤波是本实验的固有特征，因为当线宽接近耦合常数时，交叉峰幅度迅速衰减。我们在这里展示了噬菌体λ阻遏物的NH2-末端臂的灵活性，该臂被认为缠绕在阻遏物-操作符复合物中的双螺旋周围。臂共振的分配是通过构建含有连续NH2-末端缺失的突变阻遏基因而实现的。
Flexible regions of proteins play an important role in catalysis, ligand binding, and macromolecular interactions. Because of its enhanced sensitivity to motional narrowing, two-dimensional coupling constant J-correlated 1H NMR may be used to observe these regions selectively. Dynamic filtering is an intrinsic feature of this experiment because cross-peak amplitude decays rapidly as linewidths approach the coupling constant. We demonstrate here the flexibility of the NH2-terminal arm of phage lambda repressor, which is thought to wrap around the double helix in the repressor-operator complex. The assignment of arm resonances is made possible by the construction of mutant repressor genes containing successive NH2-terminal deletions.