Study on the inhibition of telomerase activity and regulated mechanism in human cancer cell by Icarrin
Study on the inhibition of telomerase activity and regulated mechanism in human cancer cell by Icarrin
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发表时间：
2002
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通讯作者：
M. Hai
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作者：
M. Hai
目的：研究淫羊藿苷（伊卡）对HL-60细胞端粒酶活性的抑制作用及其机制。方法：MTT法、NBT法、TRAP-PCR法、RT-PCR法、流式细胞术。结果：伊卡对HL-60细胞端粒酶活性有明显的抑制作用，端粒酶活性与HL-60细胞CD 11b抗原表达率呈负相关，并能诱导HL-60细胞向成熟粒细胞分化，改变HL-60细胞的细胞周期分布，表现为绝大多数细胞聚集于G 0/G1期，S期细胞丢失。结论：伊卡抑制HL-60细胞端粒酶活性的机制可能是从细胞水平的基因-蛋白-效应上进行的。
Objective:To study inhibited tecomerase activity of HL-60 cells in human cancer cell by Icarrin(ICA) and its mechanism.Methods:MTT assay,NBT assay, TRAP-PCR,RT-PCR assay,Flow Cytometry.Results:ICA inhibited telomerase activity in HL-60 cells significantly.It was negative correlation between telomerase activity and the expression ratio of CD11b antigen in HL-60 cells.It can induced HL-60 cells to differentiate into mature granulocytes.It can changed cell cycle distribution of HL-60 cells,which was reflected by the accumulation of the vast majority of cells in the G0/G1phase and the loss of cells in the S phase. It can downregulated cell proliferation related gene c-myc;upregulated p21 gene protein and mRNA expression.Conclusion:This study proved mechanism of ICA on inhibited telomerase activity from gene-protein-effect of cell level in HL-60 cells.