Chromatin binding of estradiol in the hypothalamus and cortex of male and female rats
Chromatin binding of estradiol in the hypothalamus and cortex of male and female rats
复制标题
雄性和雌性大鼠下丘脑和皮质中雌二醇的染色质结合
DOI：
10.1016/0006-8993(78)90138-5
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发表时间：
1978
期刊：
影响因子：
2.9
通讯作者：
K. L. Olsen
中科院分区：
文献类型：
--
作者：
R. E. Whalen;K. L. Olsen
性腺类固醇被认为是通过细胞质结合后基因组的激活，受体复合物易位到细胞核和染色质结合在特定的受体位点发挥作用。本研究探讨了雌、雄大鼠下丘脑和皮质细胞染色质结合雌二醇的动力学。在两种性别中，发现结合仅限于下丘脑细胞。结合的动力学不同的性别，雌二醇被保留在男性不太好。在女性下丘脑染色质结合的能力有限，竞争性抑制未标记的雌二醇。未标记的睾酮抑制雌二醇结合时，皮下注射3小时前的雌二醇，但不是由睾酮静脉注射或同时与雌二醇。这表明，下丘脑染色质结合的性别差异可能是男性对雌二醇的前凸诱导特性相对不敏感的基础。
Gonadal steroids are thought to act by activation of the genome following cytoplasmic binding, translocation of the hormone-receptor complex to the nucleus and chromatin binding at specific acceptor sites. This study examined the kinetics of chromatin binding of estradiol by hypothalamic and cortical cells of adult gonadectomized male and female rats. In both sexes binding was found to be limited to hypothalamic cells. The kinetics of binding differed between the sexes, estradiol being retained less well in males. In females hypothalamic chromatin binding was shown to be of limited capacity and to be competitively inhibited by unlabeled estradiol. Unlabeled testosterone inhibited estradiol binding when administered subcutaneously 3 h before the estradiol, but not by testosterone when administered either intravenously or concurrently with the estradiol. It was suggested that the observed sex difference in hypothalamic chromatin binding might underlie the relative insensitivity of the male to the lordosis-inducing properties of estradiol.