Activation of ventrolateral preoptic neurons by the somnogen prostaglandin D2
Activation of ventrolateral preoptic neurons by the somnogen prostaglandin D2
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DOI：
10.1073/pnas.95.13.7754
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发表时间：
1998-06-23
影响因子：
11.1
通讯作者：
Hayaishi, O
中科院分区：
文献类型：
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作者：
Scammell, T;Gerashchenko, D;Hayaishi, O
前列腺素 D-2 (PGD(2)) 是一种被广泛研究的促进睡眠的物质，但 PGD(2) 诱导睡眠的神经解剖学基础仅被部分了解。为了确定参与这种反应的潜在区域，我们使用 Fos 免疫组织化学来识别通过将 PGD(2) 注入前脑头端下方的蛛网膜下腔而激活的神经元，PGD(2) 增加非快速眼动睡眠并诱导腹外侧视前区 (VLPO) 中 Fos 的显着表达，VLPO 是一组神经元，可通过抑制结节乳头核（上行组胺能细胞的来源）来促进睡眠。在唤醒系统中，VLPO 中的 Fos 表达与前期睡眠量呈正相关，与结节乳头核中的 Fos 表达呈负相关。 PGD(2) 还增加了基底软脑膜和与自主调节有关的几个区域的 Fos 免疫反应性。这些观察结果表明，PGD(2) 可能通过软脑膜 PGD(2) 受体诱导睡眠，随后激活 VLPO。
Prostaglandin D-2 (PGD(2)) is an extensively studied sleep-promoting substance, but the neuroanatomical basis of PGD(2)-induced sleep is only partially understood. To determine potential regions involved in this response, we used Fos immunohistochemistry to identify neurons activated by infusion of PGD(2) into the subarachnoid space below the rostral basal forebrain, PGD(2) increased nonrapid eye movement sleep and induced striking expression of Fos in the ventrolateral preoptic area (VLPO), a cluster of neurons that may promote sleep by inhibiting the tuberomammillary nucleus, the source of the ascending histaminergic arousal system, Fos expression in the VLPO was positively correlated with the preceding amount of sleep and negatively correlated with Fos expression in the tuberomammillary nucleus. PGD(2) also increased Fos immunoreactivity in the basal leptomeninges and several regions implicated in autonomic regulation, These observations suggest that PGD(2) may induce sleep via leptomeningeal PGD(2) receptors with subsequent activation of the VLPO.