Förster energy transfer measurements of thiol 1 to thiol 2 distances in myosin subfragment 1.
Förster energy transfer measurements of thiol 1 to thiol 2 distances in myosin subfragment 1.
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Förster 测量肌球蛋白亚片段 1 中硫醇 1 到硫醇 2 距离的能量转移。
DOI：
10.1021/bi00289a014
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Yount,RG
中科院分区：
文献类型：
--
作者：
Dalbey,RE;Weiel,J;Yount,RG
罗斯·E放大图片作者：J. Yount* abstract：福斯特能量转移用于测量肌球蛋白SH和SH 2的两个反应性硫醇上的报告基团之间的距离，并检测核苷酸结合后该距离的变化。SH用荧光团5-[[2-[（碘乙酰基）氨基]乙基]氨基]萘-1-磺酸（1，5-IAEDANS）标记，SH 2用发色受体N-[4-（二甲氨基）-3，5-二硝基苯基]-马来酰亚胺（DDPM）标记。肽段研究证实，[~ 3 H]-1，5-IAEDANS与SH_2特异性反应，而[~（14）C] DDPM标记SH_2和碱性轻链。通过瓦格纳和Weeds [瓦格纳，P.D.，& Weeds，A. G.等（1977）J. Mol. 109，455-473]，用1，5-IAE-DANS然后用DDPM标记的亚片段1显示两种荧光
Ross E. Dalbey, Jurgen Weiel, J and Ralph G. Yount* abstract: Forster energy transfer was used to measure the distance between reporter groups on the two reactive thiols of myosin, SH, and SH2, and to detect changes in this distance upon binding of nucleotide. SH, was labeled with the fluorophore 5-[[2-[(iodoacetyl) amino] ethyl] amino] naphthalene-1-sulfonic acid (1, 5-IAEDANS) and SH2 with the chromophoric acceptor/V-[4-(dimethylamino)-3, 5-dinitrophenyl]-maleimide (DDPM). Peptide studies verified that [3H]-1, 5-IAEDANS reacted specificallywith SH,, while [14C] DDPM labeled both SH2 and the alkali light chains. The [14C]-DDPM-modified alkali light chains were replaced with un-modified light chains by the exchange procedure of Wagner and Weeds [Wagner, P. D., & Weeds, A. G.(1977) J. Mol. Biol. 109, 455-473], Subfragment 1 labeled with 1, 5-IAE-DANS and then with DDPM exhibited two fluorescence