CLONING AND CHARACTERIZATION OF A 6TH ADENYLYL CYCLASE ISOFORM - TYPE-V AND TYPE-VI CONSTITUTE A SUBGROUP WITHIN THE MAMMALIAN ADENYLYL CYCLASE FAMILY
CLONING AND CHARACTERIZATION OF A 6TH ADENYLYL CYCLASE ISOFORM - TYPE-V AND TYPE-VI CONSTITUTE A SUBGROUP WITHIN THE MAMMALIAN ADENYLYL CYCLASE FAMILY
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DOI：
10.1073/pnas.89.18.8774
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发表时间：
1992-09-15
影响因子：
11.1
通讯作者：
ISHIKAWA, Y
中科院分区：
文献类型：
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作者：
KATSUSHIKA, S;CHEN, L;ISHIKAWA, Y
哺乳动物腺苷酸环化酶家族的第六个成员已经从犬心脏cDNA文库中分离出来。这种亚型与V型的同源性比与其他腺苷酸环化酶类型的同源性更高;即使在跨膜区也存在序列相似性，其中跨膜区的类型之间存在最大的差异。VI型mRNA表达在心脏和脑中最丰富;然而，与V型不同，在检查的各种其他组织中也观察到低水平的表达。VI型腺苷酸环化酶可被NaF、鸟苷5 '-[γ-硫代]三磷酸和毛喉素刺激，但不被Ca 2 +/钙调蛋白刺激，而被腺苷及其类似物抑制。它们的结构和生化特性的比较表明，V型和VI型构成哺乳动物腺苷酸环化酶家族的一个不同的亚组。
A sixth member of the mammalian adenylyl cyclase family has been isolated from a canine cardiac cDNA library. This isoform is more highly homologous to type V than to the other adenylyl cyclase types; sequence similarity is apparent even in the transmembrane regions where the greatest divergence among the types exists. Type VI mRNA expression is most abundant in heart and brain; however, unlike type V, a low level of expression is also observed in a variety of other tissues examined. Type VI adenylyl cyclase can be stimulated by NaF, guanosine 5'-[gamma-thio]triphosphate, and forskolin but not by Ca2+/calmodulin, whereas it is inhibited by adenosine and its analogues. Comparison of both their structural and biochemical properties suggests that types V and VI constitute a distinct subgroup of the mammalian adenylyl cyclase family.