Assessment of immunotoxicity by multiparameter flow cytometry
Assessment of immunotoxicity by multiparameter flow cytometry
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DOI：
10.1006/faat.1997.2325
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发表时间：
1997-07-01
期刊：
影响因子：
--
通讯作者：
Ladics, GS
中科院分区：
文献类型：
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作者：
Burchiel, SW;Kerkvliet, NL;Ladics, GS
流式细胞术是一种独特的技术，可用于检查免疫毒性药物对免疫系统靶细胞的影响。本次研讨会的目的是概述流式细胞术在新的和已建立的免疫毒性模型中的应用，重点是潜在的应用、分析验证和潜在的缺陷。本综述首先讨论了在表面标记物定义的人B细胞、T细胞和单核细胞中评估淋巴细胞激活的Ca2+依赖机制的有用方法。本文还讨论了细胞术在细胞凋亡分析中的应用。第二篇论文介绍了如何将细胞术作为Cr-51释放试验的替代方法来评估细胞毒性T细胞活化的发展和使用数据。接下来介绍了使用表面标记来表征和区分化学刺激物和致敏剂的影响，然后概述了使用荧光探针来评估细胞硫醇状态和对淋巴样细胞的总体氧化诱导损伤。最后，一项旨在比较和评估流式细胞术在大鼠脾细胞分型中的应用的实验室间研究被提出。总的来说，这些研究证明了流式细胞术检测在免疫毒理学研究中的效用，但需要进一步的努力来验证许多这些检测在免疫毒理学检测中的常规应用。(C) 1997毒物学会。
Flow cytometry is a unique technology useful in the examination of effects of immunotoxic agents on target cells of the immune system. The purpose of this workshop was to provide an overview of the use of flow cytometry in new and established models of immunotoxicity, with emphasis on the potential applications, assay validation, and potential pitfalls. This overview begins with a discussion of methods useful in the assessment of Ca2+-dependent mechanisms of lymphoid cell activation in surface marker-defined human B cells, T cells, and monocytes. A discussion of the use of how cytometry in analysis of apoptosis is also presented in this paper. The second paper presents data on the development and use of how cytometry as an alternative to a Cr-51 release assay for an assessment of cytotoxic T cell activation. The use of surface markers for characterizing and distinguishing the effects of chemical irritants from sensitizers is next presented, followed by an overview of the use of fluorescent probes to assess cell thiol status and overall oxidant-induced injury to lymphoid cells. Finally, an interlaboratory study designed to compare and evaluate the use of flow cytometry procedures in rat splenic cell subtyping is presented. Overall, these studies demonstrate the utility of flow cytometry assays in immunotoxicologic research, but further efforts are needed in the validation of many of these assays for routine use in immunotoxicologic testing. (C) 1997 Society of Toxicology.