Effects of pertussis toxin on caudate neuron electrophysiology: studies with dopamine D1 and D2 agonists.
Effects of pertussis toxin on caudate neuron electrophysiology: studies with dopamine D1 and D2 agonists.
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百日咳毒素对尾状神经元电生理学的影响：多巴胺 D1 和 D2 激动剂的研究。
DOI：
10.1016/0006-8993(90)91348-k
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Freedman,R
中科院分区：
文献类型：
--
作者：
Bickford-Wimer,P;Kim,M;Boyajian,C;Cooper,DM;Freedman,R
选择性d1和d2激动剂SKF-38393和N-0437分别在经PT预处理的尾状核中进行测试。采用配对记录模式，对侧未处理的尾状核作为对照。用微移液管将SKF-38393和N-O437局部注射到对照组和pt预处理的尾状神经元上。在给予PT后，观察到对2受体拮抗剂的反应明显减弱。在PT侧测试的12个细胞中，只有1个对局部应用N-O437有反应，而在对照侧，90%的神经元对药物有反应。来自pt预处理和对照尾状核的神经元都对局部应用SKF-38393有反应。除了特定的d1和d2受体激动剂外，还测试了DA和间接多巴胺激动剂PCP的反应性变化。多巴胺在对照组和经pt预处理的尾状神经元中同样有效，这表明微管局部施加的多巴胺可以与pt预处理的尾状神经元中未受干扰的d1受体相互作用。另一方面，给药后对PCP的反应明显减弱，这表明内源性释放的DA优先与d2受体亚型相互作用。综上所述，这些数据表明d2受体在尾状核多巴胺反应的生理学中起着重要作用。
The selective D1and D2-agonists SKF-38393 and N-0437 respectively, were tested in caudate pretreated with PT. A paired recording paradigm was used where the contralateral untreated caudate served as a control. Micropipettes were used to locally apply SKF-38393 and N-O437 onto neurons in both control and PT-pretreated caudate. A significant attenuation of the responses to the D2agonist were observed after PT administration. Only 1 out 12 cells tested on the PT side demonstrated any response to locally applied N-O437, whereas 90% of the neurons responded to the drug on the control side. Neurons from both the PT-pretreated and control caudates responded to locally applied SKF-38393. In addition to the specific D1and D2receptor agonists, DA and the indirect dopamine agonist PCP were tested for changes in responsiveness. Dopamine was equally efficacious at both control and PT-pretreated caudate neurons, which suggest that dopamine locally applied from the micropipette can interact with the unperturbed D1receptors in the PT-pretreated caudate. On the other hand, the response to PCP was significantly attenuated after PT administration, which suggest that endogenously released DA preferentially interacts with the D2receptor subtype. Taken together these data suggest an important role for the D2receptor in the physiology of dopamine responsiveness in the caudate nucleus.