Whole influenza virus vaccine is more immunogenic than split influenza virus vaccine and induces primarily an IgG2a response in BALB/c mice
Whole influenza virus vaccine is more immunogenic than split influenza virus vaccine and induces primarily an IgG2a response in BALB/c mice
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DOI：
10.1111/j.1365-3083.2005.01633.x
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发表时间：
2005-07-01
影响因子：
3.7
通讯作者：
Haaheim, LR
中科院分区：
文献类型：
--
作者：
Hovden, AO;Cox, RJ;Haaheim, LR
本研究的目的是比较动力学和体液免疫反应的大小两种不同的流感疫苗配方，全病毒和分裂病毒疫苗。用7.5、15或30 μ g单价A/Panama/2007/99 （H3N2）病毒分裂或全病毒疫苗肌肉注射1次或2次（间隔3周）免疫BALB/c小鼠。这两种疫苗制剂诱导了相似的抗体分泌细胞反应动力学；然而，在脾脏和骨髓中观察到大小的差异。接种全病毒疫苗通常会引起更快和更高的中和抗体反应，特别是在第一剂疫苗之后。这两种疫苗制剂具有不同的免疫球蛋白G （IgG）亚类谱。分裂病毒疫苗同时刺激IgG1和IgG2a抗体，提示t -辅助性1 （Th1）和Th2混合应答，而全病毒疫苗主要诱导IgG2a抗体应答，提示Th1应答为主。全病毒疫苗在幼稚人群中免疫原性的增强可以降低提供保护性免疫所需的疫苗浓度。
The aim of this study was to compare the kinetics and the magnitude of the humoral immune response to two different influenza vaccine formulations, whole and split virus vaccines. BALB/c mice were immunized intramuscularly with one or two doses (3 weeks apart) of 7.5, 15 or 30 mu g of haemagglutinin of monovalent A/Panama/2007/99 (H3N2) split or whole virus vaccine. The two vaccine formulations induced similar kinetics of the antibody-secreting cells response; however, differences in the magnitude were observed in the spleen and bone marrow. Vaccination with whole virus vaccine generally elicited a quicker and higher neutralizing antibody response, particularly after the first dose of vaccine. The two vaccine formulations gave different immunoglobulin G (IgG) subclass profiles. Split virus vaccine stimulated both IgG1 and IgG2a antibodies suggestive of mixed T-helper 1 (Th1) and Th2 response, whereas whole virus vaccine induced mainly an IgG2a antibody response, which is indicative of a dominant Th1 response. The increased immunogenicity of whole virus vaccine in a naive population could reduce the vaccine concentration needed to provide protective immunity.