SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley.
SNP-based high density genetic map and mapping of btwd1 dwarfing gene in barley.
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基于SNP的大麦btwd1矮化基因高密度遗传图谱及定位
DOI：
10.1038/srep31741
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发表时间：
2016-08-17
影响因子：
4.6
通讯作者：
Sun D
中科院分区：
文献类型：
--
作者：
Ren X;Wang J;Liu L;Sun G;Li C;Luo H;Sun D
高密度连锁图谱是功能基因组学和育种的宝贵工具。限制酶切位点相关DNA(RAD)测序是一种新发展起来的基于序列的标记技术，已被证明是快速发现和分型全基因组单核苷酸多态(SNP)标记和构建高密度遗传图谱的有力工具。本研究的目的是利用RAD测序技术构建大麦的高密度遗传图谱。开发了1894个高质量的SNP标记，并与68个SSR标记一起定位在所有7条染色体上。这些标记的总遗传长度为1375.8 cM，相邻座位间的平均遗传长度为0.70 cM。每个连锁群内的标记数在209~396个之间。将华矮11号新的隐性矮秆基因btwd1定位到高密度连锁图谱上。结果表明，btwd1位于7H染色体上的SNP标记7HL_6335336和7_249275418之间，遗传距离分别为0.9cM和0.7cM。本研究建立的基于SNP的大麦高密度遗传图谱和矮秆基因btwd1的定位为大麦btwd1基因的定位克隆和分子育种提供了重要信息。
A high-density linkage map is a valuable tool for functional genomics and breeding. A newly developed sequence-based marker technology, restriction site associated DNA (RAD) sequencing, has been proven to be powerful for the rapid discovery and genotyping of genome-wide single nucleotide polymorphism (SNP) markers and for the high-density genetic map construction. The objective of this research was to construct a high-density genetic map of barley using RAD sequencing. 1894 high-quality SNP markers were developed and mapped onto all seven chromosomes together with 68 SSR markers. These 1962 markers constituted a total genetic length of 1375.8 cM and an average of 0.7 cM between adjacent loci. The number of markers within each linkage group ranged from 209 to 396. The new recessive dwarfing gene btwd1 in Huaai 11 was mapped onto the high density linkage maps. The result showed that the btwd1 is positioned between SNP marks 7HL_6335336 and 7_249275418 with a genetic distance of 0.9 cM and 0.7 cM on chromosome 7H, respectively. The SNP-based high-density genetic map developed and the dwarfing gene btwd1 mapped in this study provide critical information for position cloning of the btwd1 gene and molecular breeding of barley.