The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder.
The stem cell growth factor receptor KIT is not expressed on interstitial cells in bladder.
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DOI：
10.1111/jcmm.13054
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发表时间：
2017-06
影响因子：
5.3
通讯作者：
Neuhaus J
中科院分区：
文献类型：
--
作者：
Gevaert T;Ridder D;Vanstreels E;Daelemans D;Everaerts W;Aa FV;Pintelon I;Timmermans JP;Roskams T;Steiner C;Neuhaus J
肥大细胞/干细胞生长因子受体KIT长期以来被认为是膀胱Cajal间质细胞（ICC）的特异性标记物，可能具有药物前景。然而，近年来，一些作者对KIT + ICC的存在提出了质疑。因此，本研究的目的是试图澄清关于人类、大鼠、小鼠和豚鼠膀胱中KIT表达的相互矛盾的报道，并阐明抗体相关问题和物种间差异在此事中的可能作用。从人、大鼠、小鼠和豚鼠膀胱切除术中获得新鲜样品，并进行单/双免疫组织化学/免疫荧光处理。使用针对KIT、肥大细胞胰蛋白酶（MCT）、ANO1和vimentin的特异性抗体来表征表达KIT的细胞类型。以肠（空肠）组织作为外源抗体对照。我们的结果显示，KIT在人、大鼠、小鼠和豚鼠膀胱肥大细胞上表达，而在ICC上不表达。平行免疫组化显示KIT在人、大鼠、小鼠和豚鼠肠道ICC上表达，证实了KIT抗体克隆的选择性。总之，我们已经证明，人、大鼠、小鼠和豚鼠膀胱中的KIT +细胞是肥大细胞，而不是ICC细胞。本报告很重要，因为它反对KIT + ICC存在于膀胱中的观点。从这个角度来看，KIT + ICC参与膀胱生理的感觉和/或运动方面的功能概念应该被修改。
The mast/stem cell growth factor receptor KIT has long been assumed to be a specific marker for interstitial cells of Cajal (ICC) in the bladder, with possible druggable perspectives. However, several authors have challenged the presence of KIT + ICC in recent years. The aim of this study was therefore to attempt to clarify the conflicting reports on KIT expression in the bladder of human beings, rat, mouse and guinea pig and to elucidate the possible role of antibody‐related issues and interspecies differences in this matter. Fresh samples were obtained from human, rat, mouse and guinea pig cystectomies and processed for single/double immunohistochemistry/immunofluorescence. Specific antibodies against KIT, mast cell tryptase (MCT), anoctamin‐1 (ANO1) and vimentin were used to characterize the cell types expressing KIT. Gut (jejunum) tissue was used as an external antibody control. Our results revealed KIT expression on mast cells but not on ICC in human, rat, mouse and guinea pig bladder. Parallel immunohistochemistry showed KIT expression on ICC in human, rat, mouse and guinea pig gut, which confirmed the selectivity of the KIT antibody clones. In conclusion, we have shown that KIT + cells in human, rat, mouse and guinea pig bladder are mast cells and not ICC. The present report is important as it opposes the idea that KIT + ICC are present in bladder. In this perspective, functional concepts of KIT + ICC being involved in sensory and/or motor aspects of bladder physiology should be revised.