A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants
A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants
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DOI：
10.1038/nature11001
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发表时间：
2012-05-03
期刊：
影响因子：
64.8
通讯作者：
Kleine-Vehn, Juergen
中科院分区：
文献类型：
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作者：
Barbez, Elke;Kubes, Martin;Kleine-Vehn, Juergen
植物激素生长素作为一个突出的信号，通过其在选定细胞中的局部积累或消耗，为发育程序的变化提供空间和时间参考（1-7）。生长素的分布取决于生长素代谢（生物合成、接合和降解）（8-10）和细胞生长素运输（11-15）。我们在计算机上确定了一个新的假定生长素运输促进剂家族，称为PIN样（PILS）。在这里，我们说明，PILS蛋白所需的生长素依赖性调节植物生长，通过确定细胞的敏感性生长素。PILS蛋白调节细胞内生长素在内质网的积累，从而调节细胞核生长素信号传导的生长素可用性。PILS活性影响内源生长素吲哚-3-乙酸（IAA）的水平，可能是通过细胞内积累和代谢。我们的发现揭示了在细胞内将生长素区室化的运输机制具有意想不到的分子复杂性，并证明了这种区室化在许多发育过程中具有重要的功能。
The phytohormone auxin acts as a prominent signal, providing, by its local accumulation or depletion in selected cells, a spatial and temporal reference for changes in the developmental program(1-7). The distribution of auxin depends on both auxin metabolism (biosynthesis, conjugation and degradation)(8-10) and cellular auxin transport(11-15). We identified in silico a novel putative auxin transport facilitator family, called PIN-LIKES (PILS). Here we illustrate that PILS proteins are required for auxin-dependent regulation of plant growth by determining the cellular sensitivity to auxin. PILS proteins regulate intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signalling. PILS activity affects the level of endogenous auxin indole-3-acetic acid (IAA), presumably via intracellular accumulation and metabolism. Our findings reveal that the transport machinery to compartmentalize auxin within the cell is of an unexpected molecular complexity and demonstrate this compartmentalization to be functionally important for a number of developmental processes.