Standard versus accelerated riboflavin-ultraviolet corneal collagen crosslinking: Resistance against enzymatic digestion.
Standard versus accelerated riboflavin-ultraviolet corneal collagen crosslinking: Resistance against enzymatic digestion.
复制标题
DOI：
10.1016/j.jcrs.2015.10.004
复制
发表时间：
2015-09
影响因子：
2.8
通讯作者：
Meek KM
中科院分区：
文献类型：
--
作者：
Aldahlawi NH;Hayes S;O'Brart DP;Meek KM
检测标准和加速角膜胶原交联（CXL）对角膜酶抗性的影响。英国卡迪夫，卡迪夫大学视光学和视觉科学学院。实验研究。将66只摘除的猪眼（去除角膜上皮）分为6组。第1组未接受治疗，第2组接受右旋糖酐滴眼液，第3至6组接受核黄素/右旋糖酐滴眼液。第4组接受标准CXL（3 mW/cm 2紫外线A照射30分钟），而第5组和第6组接受加速CXL（分别为9 mW/cm 2照射10分钟和18 mW/cm 2照射5分钟）。对每个角膜的中心8.0 mm环钻纽扣进行胃蛋白酶消化。每天测量角膜直径，消化12天后记录每组5个样本的干重。所有CXL组（4 - 6）消化时间更长，12天时干重更大（P < .0001），而非辐照组（1 - 3）（P < .0001）。完全消化所需的时间在标准和加速CXL组之间没有差异，但12天时的干重在处理之间显示出显著差异：标准CXL 3 mW >加速CXL 9 mW >加速CXL 18 mW（P < .0001）。标准和加速CXL均增加角膜酶抗性;然而，当使用加速CXL时，CXL的量可能较少。预防疾病进展所需的CXL的精确量尚不清楚。没有作者在提到的任何材料或方法中拥有经济或所有权利益。
To examine the effect of standard and accelerated corneal collagen crosslinking (CXL) on corneal enzymatic resistance. School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom. Experimental study. Sixty-six enucleated porcine eyes (with corneal epithelium removed) were assigned to 6 groups. Group 1 remained untreated, group 2 received dextran eyedrops, and groups 3 to 6 received riboflavin/dextran eyedrops. Group 4 had standard CXL (3 mW/cm2 ultraviolet-A for 30 minutes), whereas groups 5 and 6 received accelerated CXL (9 mW/cm2 for 10 minutes and 18 mW/cm2 for 5 minutes, respectively). Trephined central 8.0 mm buttons from each cornea underwent pepsin digestion. Corneal diameter was measured daily, and the dry weight of 5 samples from each group was recorded after 12 days of digestion. All CXL groups (4 to 6) took longer to digest and had a greater dry weight at 12 days (P < .0001) than the nonirradiated groups (1 to 3) (P < .0001). The time taken for complete digestion to occur did not differ between the standard and accelerated CXL groups, but the dry weights at 12 days showed significant differences between treatments: standard CXL 3 mW > accelerated CXL 9 mW > accelerated CXL 18 mW (P < .0001). Standard and accelerated CXL both increased corneal enzymatic resistance; however, the amount of CXL might be less when accelerated CXL is used. The precise amount of CXL needed to prevent disease progression is not yet known. No author has a financial or proprietary interest in any material or method mentioned.