Effects of Temperature, Scarification, Stratification, Phytohormones, and After-Ripening on the Dormancy and Germination of Eucommia ulmoides Oliv. Seeds
Effects of Temperature, Scarification, Stratification, Phytohormones, and After-Ripening on the Dormancy and Germination of Eucommia ulmoides Oliv. Seeds
复制标题
温度、松土、层积、激素和后熟对杜仲休眠和萌发的影响。
作者：
Shiming Deng;Zhijun Deng;Xiaofeng Wang;Hai Lu;Hua Xue
杜仲、是杜仲科（Eucomiaceae）的唯一成员，为中国特有植物，具有很大的开发利用前景。E.榆属植物表现出休眠，但其潜在机制尚不清楚。本研究的目的是明确种子休眠的原因，为该物种的育种、遗传改良和种质资源的保存提供基础知识。根据Jerry M.巴斯金和卡罗尔C.巴斯金的方法，比较了完整种子和离体胚的发芽率，绘制了吸水曲线，并对划痕、冷/暖湿层积、干藏后熟和赤霉素（GA 3）处理的种子进行了发芽率测定。结果表明，完整种子仅在10 °C下萌发，萌发率较低，仅为13.3%，而离体胚在较宽的温度范围内正常萌发率较高。通过对划痕种子、半粒种子和完整种子的研究发现，种皮对胚的吸水有明显的抑制作用。划痕、后熟、冷/暖湿层积和GA 3处理促进了种子萌发。其中，冷湿层积是最有效的层积方法，随着层积时间的延长，种子萌发的温度范围从10 °C开始向两个方向增加。恒温条件下，种子发芽率显著提高，在10 °C和光照/黑暗交替条件下，经90 d冷湿层积后，种子发芽率最高，达93.7 ± 0.3%。因此，新鲜收获的E.榆种子表现出包括物理休眠和3型非深度生理休眠的组合休眠，导致种皮对胚水的吸收有限，胚生长潜力低。鉴于E.本研究结果将促进榆种子休眠演化的研究和榆属植物的开发应用。榆属种质资源
Eucommia ulmoides Oliv., the only member of the family Eucommiaceae, is endemic to China and has great development and utilization prospects. The seeds of E. ulmoides show dormancy but the underlying mechanism remains unknown. The aim of this study was to determine the cause of the seed dormancy and provide fundamental knowledge for the breeding, genetic improvement, and conservation of the germplasm resources of this species. According to the seed dormancy classification system developed by Jerry M. Baskin and Carol C. Baskin, we compared the germination percentage between intact seeds and isolated embryos, constructed water absorption curves, and evaluated the germination of seeds treated with scarification, cold/warm-moist stratification, after-ripening during dry storage, and gibberellic acid (GA3). The results showed that the intact seeds germinated only at 10 °C with a low germination percentage of 13.3% whereas the isolated embryos had a high normal germination percentage among a wider range of temperatures. According to the results from the scarified seeds, half seeds, and intact seeds, the seed coat significantly restricted the embryo water absorption. The scarification, after-ripening, cold/warm-moist stratification, and GA3 treatments promoted seed germination. Among them, cold-moist stratification was the most effective method and the temperature range of seed germination increased in both directions from 10 °C with prolonged stratification. The germination percentage increased significantly at constant temperatures with the highest germination percentage of 93.7 ± 0.3% at 10 °C and a light/dark cycle after 90 days of cold-moist stratification. Therefore, the freshly harvested E. ulmoides seeds exhibited a combinational dormancy comprising physical and Type 3 non-deep physiological dormancy, causing limited embryo water absorption by the seed coat and a low embryo growth potential. Given the unique phylogenetic characteristics and utility of E. ulmoides, our findings should promote studies of seed dormancy evolution and the development and application of E. ulmoides germplasm resources.
登录
查看更多内容
影响因子：
3.2
作者：
Jichuan Zhang;Zhaohong Xue
通讯作者：
Jichuan Zhang;Zhaohong Xue
DOI：
10.3390/plants10020235
发表时间：
2021-01-26
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Ņečajeva J;Bleidere M;Jansone Z;Gailīte A;Ruņģis D
通讯作者：
Ruņģis D
影响因子：
3
作者：
S. Manchester
通讯作者：
S. Manchester
冷层积、温度、光照、GA(3)、KNO3对云南报春种子萌发的影响
影响因子：
4.8
作者：
Yang Li-E;Peng De-Li;Li Zhi-Min;Huang Li;Yang Juan;Sun Hang
通讯作者：
Sun Hang
影响因子：
3
作者：
S. Owens;P. Fields;F. Ewers
通讯作者：
S. Owens;P. Fields;F. Ewers