Regulation of human erythroid proliferation in vitro by leukocyte surface components.
Regulation of human erythroid proliferation in vitro by leukocyte surface components.
复制标题
白细胞表面成分对体外人红细胞增殖的调节。
DOI：
10.1111/j.1749-6632.1985.tb20821.x
复制
发表时间：
1985
影响因子：
5.2
通讯作者：
Cohen,CM
中科院分区：
文献类型：
--
作者：
Dainiak,N;Cohen,CM
在适当的条件下，哺乳动物红系细胞将在原代组织培养中繁殖并合成血红蛋白。尽管通过使用化学成分确定的生长培养基在体外检查红细胞生成更容易理解导致成熟的功能性红细胞产生的复杂的体内行为，但接种的造血组织的细胞和分子复杂性掩盖了红细胞生长的特定要求的性质。因此，虽然很明显细胞相互作用是最佳红细胞生成的一个重要决定因素：“关于造血细胞和其他细胞如何在体外通信以支持红细胞生成知之甚少，关于局部骨髓环境中的这种调节的信息甚至更少。白细胞代表一种位于体内发育中的红系细胞附近的细胞类型。它们赋予组织培养基一种生长因子，该生长因子对于红系爆发形成单位（BFU-ES）的存活和增殖是必需的。我们通过询问循环中的白细胞如何释放生长促进分子来研究一种类型的细胞相互作用，即白细胞-红细胞相互作用的特征，当考虑哺乳动物细胞在孵育过程中将生物活性物质释放到液体培养基中的方式时，三种潜在的机制浮现在脑海中（见图1）。首先，它们可以通过囊泡与表面膜融合的过程（胞吐作用）排出已包装在胞内囊泡中的大分子。其次，它们可能以囊泡的形式从细胞表面脱落或剥落膜成分，其中可能含有少量的细胞质，
Given appropriate conditions, mammalian erythroid cells will multiply and synthesize hemoglobin in primary tissue culture. Although the complicated in vivo behavior that results in the production of mature, functional erythrocytes is more readily understood by examining erythropoiesis in vitro using chemically defined growth media,'-'cellular and molecular complexities of seeded hematopoietic tissue obscure the nature of specific requirements for erythroid growth. Thus, while it is apparent that cellular interactions are one important determinant of optimal erythropoiesis:" little is known about how hematopoietic and other cells communicate to support erythropoiesis in vitro, and even less information is available regarding such regulation within the local bone marrow environment. Leukocytes represent one cell type located in proximity to developing erythroid cells in vivo. They impart to tissue culture medium a growth factor that is essential for survival and proliferation of erythroid burst forming units (BFU-ES).~ We approached the characterization of one type of cellular interaction, the leukocyte-erythroid interaction, by asking how circulating leukocytes might release growth promoting molecules.When considering the manner in which mammalian cells release biologically active materials into liquid medium during incubation, three potential mechanisms come to mind (see FIGURE 1). First, they may eject macromolecules that have been packaged in intracellular vesicles by the process of vesicle fusion with the surface membrane (exocytosis). Second, they may shed or exfoliate membrane components from the cell surface in the form of vesicles that may contain small quantities of cytoplasmic