Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, China
Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, China
复制标题
中国新疆牛分枝杆菌的 Spoligotyping 和 MIRU-VNTR 评估。
DOI：
10.1016/j.rvsc.2011.03.012
复制
发表时间：
2012-04-01
影响因子：
2.4
通讯作者：
Li, Chuanyou
中科院分区：
文献类型：
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作者：
Sun, Zhaogang;Cao, Rui;Li, Chuanyou
牛结核分枝杆菌（Mycobacterium bovis）是牛结核病（bovine tuberculosis，bTB）的病原体，也是人类和其他哺乳动物的病原体。在这项研究中，406头奶牛筛选bTB的单一皮内比较宫颈结核菌素（SICCT）试验和IFN-γ测定。135海里从新疆维吾尔自治区31头SICCT和IFN-γ双阳性奶牛中分离到牛源性Bovis。采用Spoligotyping和MIRU-VNTR进行基因分型，分别鉴定出4种和7种基因型。9个MIRU-VNTR位点的新组合对M.新疆黄牛无性系。有趣的是，在本研究中发现了两个新的spoligotypes（SB 1903和SB 1904）和三个位点的特殊重复数（ETR-D，QUB 1895和QUB 3336）。这些结果表明，在中国新疆，一个特定的流行保守。M.从无牛结核病国家引进的亲牛群中分离到具有独特基因型的牛结核病菌株，表明可能由新疆本地品种褐牛传播。(C)2011爱思唯尔有限公司保留所有权利。
Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, is also a pathogen for human and other mammals. In this study, 406 cows were screened for bTB by both single intradermal comparative cervical tuberculin (SICCT) test and IFN-gamma assay. 135 M. bovis were isolated from 31 SICCT and IFN-gamma double-positive cows in Xinjiang Uygur Autonomous Region of China. Spoligotyping and MIRU-VNTR were evaluated for genotyping, and 4 and 7 genotypes were identified, respectively. A new combination of nine MIRU-VNTR loci was most discriminative for M. bovis clones from Xinjiang. Interestingly, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of three loci (ETR-D, QUB 1895 and QUB 3336) were discovered in this study. These results indicated a specific epidemic conservation in Xinjiang, China. M. bovis strains with the unique genotypes were isolated from the herds maintaining parent cows imported from the bTB-free countries, suggesting a possible transmission from the local breed of Xinjiang brown cattle. (C) 2011 Elsevier Ltd. All rights reserved.